Metabarcode data
Overall, 12,621,754 raw reads were obtained, distributed across 104 libraries, of which 1,405,224 passed initial cleaning and denoising steps and were classified as Acari, Collembola, and Coleoptera, resulting in 19,304 ASVs. Of these, 1,813 ASVs (1,278,294 reads) passed metaMATE filtering, applying parameters to maximize the number of surviving va-ASVs while limiting na-ASVs to comprise ≤5% of the final dataset. Parameters used and estimated contributions of a-ASVs and na-ASVs to the filtered dataset are provided in Table 1. Final filtering to remove records with less than five reads in a library resulted in the retention of 1791 ASVs (i.e. 98.7%) in the final community dataset. Summary data per library is provided in supplementary table S4, and the final set of ASVs and the community table is provided as a supplementary file.