Metabarcode data
Overall, 12,621,754 raw reads were obtained, distributed across 104
libraries, of which 1,405,224 passed initial cleaning and denoising
steps and were classified as Acari, Collembola, and Coleoptera,
resulting in 19,304 ASVs. Of these, 1,813 ASVs (1,278,294 reads) passed
metaMATE filtering, applying parameters to maximize the number of
surviving va-ASVs while limiting na-ASVs to comprise ≤5% of the final
dataset. Parameters used and estimated contributions of a-ASVs and
na-ASVs to the filtered dataset are provided in Table 1. Final filtering
to remove records with less than five reads in a library resulted in the
retention of 1791 ASVs (i.e. 98.7%) in the final community dataset.
Summary data per library is provided in supplementary table S4, and the
final set of ASVs and the community table is provided as a supplementary
file.