Traditional Indian medicine practice (Ayurveda) emphasized the role of “panchgavya” five products from Bos indicus for human welfare. Ayurveda classics, “Sushruta Samhita,” “Ashtanga Sangraha,” alluded to the therapeutic potential of pristine cow urine (CU) as drug or drug ingredients. Compelling evidence is exhibiting the innumerable medicinal properties of CU; accordingly, this elixir can directly treat complex ailments such as leprosy, tuberculosis, and fever. Also, the classics narrated many formulations that have utilized CU for the preparation of drugs, supplemented to enhance the potency. This practice is more empirical, and only a few experimental evidence is supporting the claim are known. The associated mechanisms are poorly understood and so rendered its appeal to the limited mass. The study aims to investigate bio-enhancer like properties of CU towards network pharmacology. For that, 25 medicines having anti-bacterial, anti-fungal, anti-viral, enzyme inhibitors, and anti-inflammatory actions selected as a reference. Network analysis for twenty chemotypes found in CU was carried out. First, through enrichment analysis, the KEGG and GO terms were obtained. Second, we performed protein-protein interaction studies to screen more targets. Towards this, the drug-protein and CU- protein interactions networks built separately and processed.