Fluorescence immunomicroscopy
The immunofluorescence staining of cell surface or intracellular proteins was performed as above. Thereafter, cells were resuspended in 20 µL PBS after twice washing steps with PBS and covered on a slide with a thin layer. After air-drying, cell layers were added with mounting solution (Termo Fische Scientefic, USA) and covered by coverslips for fluorescence microscopy. Images were made at a magnification of 600×.