Acknowledgements
Funding: The authors declare that they received no funding for the present study.
Conflict of interest: The authors declare that they have no conflict of interest.
Ethical approval: All procedures performed in this study were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed consent:   Informed consent was obtained from all individual participants included in the study.