METHODS
Animals
Male mice, 8 weeks old, were housed individually at temperature- and
humidity-controlled laboratory conditions
(21±1°C, 55±10%) maintained with
food and water ad libitum. Mice were tested during the dark phase of a
reverse light cycle (lights off at 8.00h and on at 20.00h).Plcb1+/- mice in a C57BL/6J background and their wild-type (WT)
littermates were used (Kim et al., 1997). All experimental protocols
were performed in accordance with the guidelines of the European
Communities Council Directive 2010/63/EU and approved by the local
ethics committee (Comitè Ètic d’Experimentació Animal-Parc de Recerca
Biomèdica de Barcelona, CEEA-PRBB, agreement N°9213). In agreement,
maximal efforts were made to reduce the suffering and the number of mice
used.