Statistical analysis
Three-way ANOVA with repeated measures was used to test the evolution
over sessions or days. Sessions or days were used as within-subject
factors and genotype (Plcb1+/- or WT) and drug (cocaine or
saline) were used as between-subjects factors. Post-hoc analyses
(Newman-Keuls) were performed when required. Comparisons between two
groups were analyzed by Student t -test or U -Mann-Whitney
depending on the distribution defined by the Kolmogorov-Smirnov
normality test and the sample size. The chi-square analyses were
performed to compare the percentage of mice that acquired operant
learning criteria in the different experimental groups. Results are
expressed as mean ± SEM or individual values with the median and the
interquartile range specified in the figure legend. Differences were
considered significant at P <0.05. The sample size was
calculated based on power analysis. The statistical analyses were
performed using the Statistical Package for Social Science tool
SPSS®25.0 (SPSS Inc, Chicago, USA).
See supplementary information for more details of genotyping of
transgenic mice, drugs, behavioral tests of phenotype characterization,
operant conditioning maintained by cocaine, RNA extraction and
sequencing.