2.9 Statistical analysis
SPSS 19 (SPSS Science Inc., Chicago, Illinois) and Prism 6.0 (Graph Pad Software, La Jolla, CA, USA) were used to perform data analysis. Quantitative data expressed as the mean ± S.E.M. Two-tailed Student’st -test was used for comparisons between the control and treated groups, and studies involving more than two groups, data were evaluated with one-way Analysis of Variance (ANOVA) followed by the Tukey’s post hoc test. Statistical significance was defined asP< 0.05.