The effect of aluminium is not linked with cytotoxicity
Since actin bundling is often heralding programmed cell death (Chang et
al., 2011), we wondered, whether treatment with 200 μM
Al3+ would increase mortality, using the Evans Blue
dye exclusion assay. However, we failed to see any significant
difference to the mock control (Figure S4 ). Mortality was
around 10% in both cases, when scored 24 h after onset of the
treatment. Thus, the aluminium treatment triggered actin remodeling
without inducing any programmed cell death.