The effect of aluminium is not linked with cytotoxicity
Since actin bundling is often heralding programmed cell death (Chang et al., 2011), we wondered, whether treatment with 200 μM Al3+ would increase mortality, using the Evans Blue dye exclusion assay. However, we failed to see any significant difference to the mock control (Figure S4 ). Mortality was around 10% in both cases, when scored 24 h after onset of the treatment. Thus, the aluminium treatment triggered actin remodeling without inducing any programmed cell death.