Figure 4
(A) Non-metric multidimensional scaling (nMDS) ordination of Kirtland’s
Warbler gut microbiome community by sampling period, compared using
Bray-Curtis distances. Ellipses show 95% confidence intervals around
the centroid of each sampling period. Three outliers were removed from
ordination plot for visualization purposes, plot including outliers is
shown in Supplemental Figure 2. (B) Ordination of individual birds
sampled in triplicate placed within the nMDS space of all samples,
highlighting intra-individual change over time.
Figure S1 Alpha and beta diversity plots of rarefied data. All
libraries were rarefied to a depth of 7,000 sequences. Rarefaction
resulted in the loss of 319 ASVs (4.3% of total ASVs). Alpha and beta
diversity analyses were performed. All results were qualitatively
similar to non-rarefied data; no variable or category gained or lost
statistical significance compared to non-rarefied data. (A) Boxplots
illustrating the relative abundance of the top five most common phyla
per individual by sampling period representing the change in relative
abundance from Cat Island, The Bahamas (CIB) to the first Michigan
recapture period (MI1) and the second Michigan recapture period (MI2).
(B) Changes in alpha diversity across the sampling periods. For plots A
and B significance levels are pairwise comparisons between sampling
periods are shown (ns: p>0.05; * p<0.05; **
p<0.01; *** p<0.001; **** p<0.0001). (C)
Non-metric multidimensional scaling (nMDS) ordination of Kirtland’s
Warbler gut microbiome community by sampling period, compared using
Bray-Curtis distances. Ellipses show 95% confidence intervals around
each sampling period.
Figure S2 – Non-metric multidimensional scaling (nMDS)
ordination of Kirtland’s Warbler gut microbiome community by sampling
period, compared using Bray-Curtis distances and including outliers
omitted in Figure 4. Ellipses show 95% confidence intervals around the
centroid of each sampling period.
Figure S3 Comparison of beta diversity measures for individuals
captured at all three sampling periods. Each line represents one
individual connecting the Bray-Curtis measurements between the first and
second sampling period to the measurement between the second and third
sampling period.