Figure 4
(A) Non-metric multidimensional scaling (nMDS) ordination of Kirtland’s Warbler gut microbiome community by sampling period, compared using Bray-Curtis distances. Ellipses show 95% confidence intervals around the centroid of each sampling period. Three outliers were removed from ordination plot for visualization purposes, plot including outliers is shown in Supplemental Figure 2. (B) Ordination of individual birds sampled in triplicate placed within the nMDS space of all samples, highlighting intra-individual change over time.
Figure S1 Alpha and beta diversity plots of rarefied data. All libraries were rarefied to a depth of 7,000 sequences. Rarefaction resulted in the loss of 319 ASVs (4.3% of total ASVs). Alpha and beta diversity analyses were performed. All results were qualitatively similar to non-rarefied data; no variable or category gained or lost statistical significance compared to non-rarefied data. (A) Boxplots illustrating the relative abundance of the top five most common phyla per individual by sampling period representing the change in relative abundance from Cat Island, The Bahamas (CIB) to the first Michigan recapture period (MI1) and the second Michigan recapture period (MI2). (B) Changes in alpha diversity across the sampling periods. For plots A and B significance levels are pairwise comparisons between sampling periods are shown (ns: p>0.05; * p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001). (C) Non-metric multidimensional scaling (nMDS) ordination of Kirtland’s Warbler gut microbiome community by sampling period, compared using Bray-Curtis distances. Ellipses show 95% confidence intervals around each sampling period.
Figure S2 – Non-metric multidimensional scaling (nMDS) ordination of Kirtland’s Warbler gut microbiome community by sampling period, compared using Bray-Curtis distances and including outliers omitted in Figure 4. Ellipses show 95% confidence intervals around the centroid of each sampling period.
Figure S3 Comparison of beta diversity measures for individuals captured at all three sampling periods. Each line represents one individual connecting the Bray-Curtis measurements between the first and second sampling period to the measurement between the second and third sampling period.