Figure 4. REVIGO Gene Ontology treemaps for TPP-identified proteins as the targets of celecoxib in the rat hippocampus. Each rectangle is a single cluster representative of enriched annotations. The loosely related annotations are joined together and visualized with different colors. The size of the rectangles was adjusted to reflect the frequency of the GO annotations in the (A) BP, (B) MF, and (C) CC.
The enriched PTMs in TPP-identified proteins were also evaluated by PEIMAN software. It is presumed that soluble proteins at 67C might be enriched in any of PTMs to last longer under temperature changes. We observed fifteen enriched PTMs, which emphasizes the role of PTMs in the thermostability of proteins. For example, Acetylation, prenylation, and phosphorylation are significantly detected in all TPP-identified proteins. Citrullination was the specific PTM for Celecoxib targets which was statistically enriched by adjusted p-value 7.6E-3. All of the enriched PTMs were confirmed by re-searching the proteomic data using these PTM as variable modifications in Proteome Discoverer.