Figure 4. REVIGO Gene Ontology treemaps for TPP-identified
proteins as the targets of celecoxib in the rat hippocampus. Each
rectangle is a single cluster representative of enriched annotations.
The loosely related annotations are joined together and visualized with
different colors. The size of the rectangles was adjusted to reflect the
frequency of the GO annotations in the (A) BP, (B) MF, and (C) CC.
The enriched PTMs in TPP-identified proteins were also evaluated by
PEIMAN software. It is presumed that soluble proteins at 67C might be
enriched in any of PTMs to last longer under temperature changes. We
observed fifteen enriched PTMs, which emphasizes the role of PTMs in the
thermostability of proteins. For example, Acetylation, prenylation, and
phosphorylation are significantly detected in all TPP-identified
proteins. Citrullination was the specific PTM for Celecoxib targets
which was statistically enriched by adjusted p-value 7.6E-3. All of the
enriched PTMs were confirmed by re-searching the proteomic data using
these PTM as variable modifications in Proteome Discoverer.