Figure 4. Change of Potential Energy during the Initializations and Optimization.
The change of potential energy during the initial structure generation and further iterative optimization is shown in blue line. The potential energy shows drastic fluctuations during the initialization of cotranslational folding which partly indicates that addition of amino acid is not always either favorable or unfavorable. This fluctuation is different from following iterative optimizations reflecting the strong effect of the change of configurations. During the optimization processes, the potential energy decreased with saltatory tendency. After three iterations of optimizations, the potential energy remained as being rather conserved indicating the fast convergence of the algorithm to the local energy minima.