Metabolite assays
Fumarate, malate and starch concentrations were estimated as previously
described (Dyson et al ., 2016). Averages and standard errors of
3-4 replicates were calculated, and an analysis of variance (ANOVA)
followed by a Turkey’s test was performed in R, with a confidence level
of 0.95. Where measurements were subtracted from one another, the
uncertainty (μ ) was calculated such that\(\mu_{X,Y}=\sqrt{\mu_{X}^{2}+\mu_{Y}^{2}}\). Measurements where the
uncertainty ranges do not overlap are considered to be significantly
different.