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\begin{document}

\title{Novel Genetic Characterization of Porcine Epidemic Diarrhoea Virus
Strains Circulating in Guangdong, China}



\author[1]{Shengnan Chen}%
\author[1]{Guangzhi Yan}%
\author[1]{Tian Tang}%
\author[1]{Rusen Deng}%
\author[1]{Xiao qing Yuan}%
\author[1]{Jiedan Liao}%
\author[1]{Xing-kui Si}%
\author[1]{Zongji Lu}%
\author[2]{Ningyi Jin}%
\author[1]{Hao Liu}%
\author[1]{Liangzong Huang}%
\affil[1]{Foshan University}%
\affil[2]{Academy of Military Sciences}%


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  \date{May 08, 2024}


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\begin{abstract}
In recent years, the emergence and high prevalence of porcine epidemic
diarrhoea virus (PEDV) in piglets has been observed in various regions
of Guangdong Province. In this study, novel genetic features of the PEDV
S1 gene were detected in fifty-five PEDV samples from eleven different
pig farms collected from 2018 to 2019 in eight cities in Guangdong
Province, China. More than 98.2\% (54/55) of the samples tested positive
with a PEDV antigen assay. The S1 gene of 11 samples was sequenced.
Analysis results showed that the four novel PEDV isolates were
99.9\%\textasciitilde{}100\% identical to each other and clustered to a
separate clade in the G2a subtype, sharing 90.4--98.8\% and 87.7--97.7\%
identities at the nucleotide and amino acid levels, respectively, with
57 strains from GenBank. It is worth noting that the novel PEDV strains
contained nine novel amino acid substitutions (L299I, V312A, Q319P,
N/D360A, S558L, S566K, K593R, Y612H and S773F) in the S1 protein
compared to the sequences of widely used vaccine strains (CV777 and
AJ1102 strains) and other variant PEDV strains (G2 group strains).
Furthermore, the amino acid substitutions were in the COE region of the
important S protein neutralization epitope. In this study, we detected
novel PEDV epidemic strains in Guangdong Province, which had the highest
identity (98.7-98.8\% nucleotide level, 96.6-97.2\% amino acid level)
with the BJ2011-1 strain and differed greatly from vaccine strains.
Compared to vaccine strains, there are 91 (CV777) or 27 (AJ1102) amino
acid changes in the neutralization epitope of the S1 protein, and
whether amino acid substitutions affect the immune efficacy of the
vaccine should be verified in further studies.%
\end{abstract}%



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