3.5 Infectious pathogens detected by mNGS
The next-generation sequencing was performed from blocks of resected lung samples for each patient. All the six blocks were available. In the current study, mNGS successfully identified the infectious pathogens in all patients, and the pathogens detected were showed in Table 2. Streptococcus pneumonia (specific reads: 941) was detected in patient No.1, whose infectious symptoms were present when surgery. Patient No.5 has suffered the disease Langerhans cell histiocytosis (LCH) in the left submandibular lymph node for 1 year, and received the suggested chemotherapy treatment four times, she had suffered recurrent pneumonia for 3 months. Neisseria mucosa (specific reads: 587), Neisseria sicca (specific reads: 247), Prevotel lamelaninogenica (specific reads: 258), and Prevotella histicola (specific reads: 174) and Fusobacterium nucleatum (specific reads: 239) were detected in case 5, which may be associated with the chemotherapy treatment for LCH. No infectious pathogen was detected in other 66.7% (4/6) cases (patient No.2 to No.4, and No. 6), whose infectious symptoms were controlled with suggested therapy before surgery.