2.4. Extraction and estimation of lipids
The standard protocol of Bligh & Dyer (1959) was followed for lipid
extraction from the drum dried microalgal biomass with chloroform,
methanol and distilled water as solvents. The organic layer was
evaporated in a water bath followed by drying at 104 °C for 30 min. The
weight of the lipid was estimated in a high precsion balace of
Sartorious, Germanny.