Figure legends
Figure 1. In vitro growth and maturation of mast cells over a 70
days period. CD34-positive peripheral blood cells from healthy
volunteers (n= 13) or individuals with indolent systemic mastocytosis
(n=14) were cultured under conditions that promote mast cell
development. Mean ± SEM.
Figure 2. Release of histamine and PGD2 from activatedin vitro developed mast cells. Mast cells were treated for 30
minutes with calcium ionophore A23187, morphine, anti-IgE or mannitol
and the release of histamine (A) and PGD2 (B) was
measured in the cell free supernatant. Healthy volunteers (open boxes)
(n=6-10) and systemic mastocytosis (filled boxes) (n=7-13). Results are
shown as box and whiskers; the box extends from the 25th to 75th
percentiles and the whiskers min to max. * p<0.05