HBO exposure
An HBO exposure experiment was performed in a temperature-adjustable hyperbaric chamber provided by the neurosurgery department of Southwest Hospital (Chongqing, China). The main parameters for HBO administration were 2.5 atmospheres absolute (ATA) of pure oxygen and a time of 120 min, and the program included 15 min of compression and decompression at a constant velocity. The temperature for cells was 37 °C, while that for animals was 25 °C. Cells in different plates were removed from the chamber after HBO exposure and cultured routinely in an incubator.