In vivo IR-780+HBO evaluation
In vivo,
treatment
was administered on the fifth day of
transplanted
tumor establishment through intraperitoneal injection of 3 mg/kg IR-780.
The IR-780+HBO group and the HBO-only group were then placed in the
hyperbaric chamber 4 h after IR-780 injection. The change in
tumor
volume was
monitored
every other day during the experiment.
After
treatment every two days for a total of 5 interventions, the mice were
weighed and sacrificed, and the transplanted tumors from the animals
were photographed and weighed. Subsequently, the tumor tissues and
organs were dissected and fixed in 4% paraformaldehyde.
Hematoxylin
and eosin (H.E.) staining
was
performed to observe the toxicity in normal tissues. To study the
long-term development of tumors after IR-780+HBO treatment, tumor volume
was monitored in the IR-780+HBO group and DDP group. After 5 injections
of
3
mg/kg IR-780 and 3 mg/kg DDP, the mice continued to be fed for 28 days.
To
compare the anti-tumor effect of IR-780+HBO and DDP on drug-resistant
bladder cancer, 3 mg/kg IR-780 and 3 mg/kg DDP were injected into
transplanted tumor mice in the IR-780 group and DDP group respectively.
After treatment every two days for a total of 5 interventions, the mice
were photographed and sacrificed, and the transplanted tumor from the
animals were photographed and weighed.