Optical imaging
In vivo fluorescence imaging mice bearing T24, MB49 transplanted tumors were sacrificed at 24 h (n=5 each) after intraperitoneal injection with IR-780 at a dose of 1 mg/kg. The dissected organs were subjected to NIR imaging with a Kodak In-Vivo FX Professional Imaging System (New Haven, CT). Cellular dye uptake and the subcellular localization cells of different cell lines (T24, MB49, HK2, and SV-HUC-1) were seeded at the same densities in culture dishes and cultured for 24 h. Then, the cells were incubated with 2 μM IR-780 in 1640 culture medium for 15 min in the incubator before being incubated for 10 min with 1 μM MitoTracker Green. Hoechst was used to stain the nuclei. Finally, these cells were imaged under a confocal microscope.