Cell lines
Human bladder cell lines (T24, 5637, and TCCSUP) were purchased from the ATCC (Manassas, VA,
USA), and human normal cell lines (HK2 and SV-HUC-1) were obtained from the Chinese Academy of Sciences Cell Bank (Shanghai, China). A mouse bladder cell line (MB49) was donated by Dr. Chunmeng Shi from the Institute of Rocket Force Medicine, Third Military Medical University (Chongqing, China). All cells were cultured in their recommended medium supplemented with 10% FBS (Gibco, USA) and 1% penicillin/streptomycin (Beyotime). All cells were passaged at 1:3 ratios and cultured in an incubator at 37 °C with 5% CO2. T24/DDP, a cisplatin-resistant bladder cancer cell line, was established by a concentration gradient method.
Methods