Colony formation assay
After intervention for 6 h, cells were seeded in six-well plates at a
density of 1000 cells per well and cultured overnight to
attach.
The medium was replaced with fresh medium after 24 h, and the cells were
cultured for 12 days.
Subsequently,
the cells were fixed with paraformaldehyde for 10 min and subjected to
crystal violet (Beyotime) staining for 5 min. The samples were
photographed, and the colonies > 0.2 mm in diameter were
counted under a
microscope.