Optical imaging
In vivo fluorescence imaging mice bearing T24, MB49 transplanted tumors
were sacrificed at 24 h (n=5 each) after
intraperitoneal
injection with IR-780 at a dose of 1
mg/kg.
The dissected organs were subjected to NIR imaging with a Kodak In-Vivo
FX Professional Imaging System (New Haven,
CT).
Cellular dye uptake and the subcellular localization cells of different
cell lines (T24, MB49, HK2, and SV-HUC-1) were seeded at the same
densities in culture dishes and cultured for 24 h. Then, the cells were
incubated with 2 μM IR-780 in 1640 culture medium for 15 min in the
incubator before being incubated for 10 min with 1 μM MitoTracker Green.
Hoechst was used to stain the nuclei. Finally, these cells were imaged
under a confocal microscope.