In vivo IR-780+HBO evaluation
In vivo, treatment was administered on the fifth day of transplanted tumor establishment through intraperitoneal injection of 3 mg/kg IR-780. The IR-780+HBO group and the HBO-only group were then placed in the hyperbaric chamber 4 h after IR-780 injection. The change in tumor volume was monitored every other day during the experiment. After treatment every two days for a total of 5 interventions, the mice were weighed and sacrificed, and the transplanted tumors from the animals were photographed and weighed. Subsequently, the tumor tissues and organs were dissected and fixed in 4% paraformaldehyde. Hematoxylin and eosin (H.E.) staining was performed to observe the toxicity in normal tissues. To study the long-term development of tumors after IR-780+HBO treatment, tumor volume was monitored in the IR-780+HBO group and DDP group. After 5 injections of 3 mg/kg IR-780 and 3 mg/kg DDP, the mice continued to be fed for 28 days. To compare the anti-tumor effect of IR-780+HBO and DDP on drug-resistant bladder cancer, 3 mg/kg IR-780 and 3 mg/kg DDP were injected into transplanted tumor mice in the IR-780 group and DDP group respectively. After treatment every two days for a total of 5 interventions, the mice were photographed and sacrificed, and the transplanted tumor from the animals were photographed and weighed.