Cell viability evaluation
Bladder cancer cell lines of different grades (T24, 5637, TCCSUP, T24/DDP and MB49) were treated with the indicated doses of IR-780 with or without HBO exposure, and a CCK-8 kit (Dojindo Kumamoto, Japan) was used to measure cell proliferation after 48 h. T24 cells after treatment with or without different inhibitors were placed in 7.5 μM IR-780+HBO, and cell viability was detected after 48 h.