Cell lines
Human bladder cell lines
(T24,
5637, and TCCSUP) were purchased from the ATCC (Manassas, VA,
USA), and human normal cell lines
(HK2
and SV-HUC-1) were obtained from the
Chinese
Academy of Sciences Cell Bank (Shanghai, China). A mouse bladder cell
line (MB49)
was
donated by Dr. Chunmeng Shi from the Institute of Rocket Force Medicine,
Third Military Medical University (Chongqing, China). All cells were
cultured in their recommended medium supplemented with 10%
FBS
(Gibco, USA) and 1% penicillin/streptomycin (Beyotime). All
cells
were passaged at 1:3 ratios and cultured in an incubator at 37 °C with
5% CO2. T24/DDP, a cisplatin-resistant bladder cancer
cell line, was established by a concentration gradient method.
Methods