HBO exposure
An HBO exposure experiment was performed in a temperature-adjustable
hyperbaric chamber provided by the neurosurgery department of Southwest
Hospital (Chongqing, China). The main parameters for HBO administration
were 2.5
atmospheres
absolute (ATA) of pure oxygen and a time of 120 min, and the program
included 15 min of compression and decompression at a constant velocity.
The
temperature for cells was
37
°C, while that for animals was 25 °C. Cells in different plates were
removed from the chamber after HBO exposure and cultured routinely in
an
incubator.