Materials
IR-780 iodide,
2-thenoyltrifluoroacetone
(TTFA),
diphenyleneiodonium
chloride (DPI),
H2O2,
and
N-acetylcysteine
(NAC) were obtained
from
Sigma-Aldrich
(St. Louis, MO, USA).
Cis-diamminedichloroplatinum
(DDP) and doxorubicin hydrochloride (DOX) were obtained from Aladdin
(Shanghai, China).
MitoTracker
Green, mitoSOX,
and
the mitochondrial membrane potential indicator
TMRM
were purchased
from
Invitrogen (Carlsbad, CA, USA). A Complex I Enzyme Activity Microplate
Assay
was purchased
from
Abcam
(Cambridge,
MA, USA). DiBAC4(3) was purchased
from
MedChem Express (Monmouth Junction, NJ, USA). A
Reactive
Oxygen Species (ROS) Assay Kit, Hoechst 33258, Crystal Violet Staining
Solution, and an ATP Assay Kit were obtained from Beyotime (Shanghai,
China).
Primary
antibodies were Rb anti-Cytochrome C (1:1000, CST, 11940T), Rb
anti-Ndufs1 (1:1000, abcam, ab169540), Rb anti-Caspase-9 (1:1000, abcam,
ab202068), Rb anti-Caspase 3 Antibody (1:1000, proteintech, 19677-1-AP),
Rb anti-cleaved Caspase 3 Antibody (1:1000, abcam, ab13847), Rb
anti-PARP (1:1000, proteintech, 13371-1-AP) and Ms anti-β-action
(1:5000, proteintech, 6008-1-Ig).