Tumor models
MB49 cancer cells grown to 70% confluence were suspended in phosphate-buffered saline (PBS). MB49 transplanting tumor model were established in female C57 BL/6 mice (6-8 weeks old and weighing 17-20 g) by subcutaneously implanting each mouse with 1*107 cells. T24, T24/DDP tumor xenografts were established in female nude mice in the same way.