Western blot analysis
Cells were harvested after treatment for 24 h, and total proteins were extracted with RIPA lysis buffer (Beyotime). After separation using 12% SDS-polyacrylamide gels, the proteins were transferred onto PVDF membranes (Millipore, USA). The PVDF membranes were blocked with Quick Blocking Buffer (Beyotime) for 15 min and then incubated overnight with primary antibodies at 4 °C. The antibodies described above were used. After incubation for 1 h with secondary antibodies in an incubator at 37 °C, the signals were identified by chemiluminescence detection.