2.2 Plasmid Construction and Plant Transformation
Two target sequences were designed in the fifth exon of OsLHYgene using the web tool CRISPR-P
(http://cbi.hzau.edu.cn/crispr/)
(Lei et al., 2014). The original CRISPR/Cas9 vector was kindly provided
by Prof. Yao-Guang Liu from South China Agricultural University.
CRISPR/Cas9 was constructed according to the protocol previously
described (Ma et al., 2015). Then Agrobacterium -mediated
transformation was performed to introduce the binary constructs into
EHA105 and rice callus generated from WT. The targets are listed in
Supplemental Table 1.