2.3 DNA extraction and mutation type detection
Genomic DNA was extracted from the leaves of rice transgenic plants using the CTAB method (Murray & Thompson, 1980). The DNA containing the targeted sequence regions was amplified and sequenced and relative primers are listed in Supplemental Table 1. The genotypes of targeted mutations were further analyzed by using DSDecode, the web-based tool (http://skl.scau.edu.cn/dsdecode/) (Liu et al., 2015).