2.2 Plasmid Construction and Plant Transformation
Two target sequences were designed in the fifth exon of OsLHYgene using the web tool CRISPR-P (http://cbi.hzau.edu.cn/crispr/) (Lei et al., 2014). The original CRISPR/Cas9 vector was kindly provided by Prof. Yao-Guang Liu from South China Agricultural University. CRISPR/Cas9 was constructed according to the protocol previously described (Ma et al., 2015). Then Agrobacterium -mediated transformation was performed to introduce the binary constructs into EHA105 and rice callus generated from WT. The targets are listed in Supplemental Table 1.