DNA extraction kits, Sequencing method, and Taxonomy
classification
A variety of approaches were employed for DNA extraction; custom
developed protocols, along with two standard extraction kits
(Table 1 ) were used in studies reporting GBS within the vaginal
microbiome. However, these kits and custom approaches were also used in
studies that did not report GBS. In terms of sequencing methods, all
studies reporting GBS used 16S rRNA sequencing, as well as most studies
that did not report the presence of GBS. Additionally, there was no
notable difference found in the 16S rRNA hypervariable region used
across either set of studies. Similarly, multiple taxonomy databases
were used for studies that did and did not report the presence of GBS.