2.10 | RNA-seq analysis
Total RNA was isolated from LF fibroblasts collected from LDH and LSCS
patients using RNeasy Plus Micro-Kit (Takara, Dalian, China) following
the manufacturer’s instructions. Then, total RNA was provided to Nanjing
medical university for preparing RNA-seq libraries and sequencing. The
sequencing libraries were generated using the TruSeq RNA Sample Prep Kit
(Illumina) as described by the manufacturer’s recommendations. The
libraries were sequenced on two lanes of an Illumina HiSeq X-Ten for
pair-end. EdgeR was used to distinguish the differentially expressing
genes by pairwise comparisons.