2.6 | Cell viability assays
LF Cells were seeded in 96-well plates at 105 cells per well and cultured at 37℃ under 5% CO2 for 24 h. Then, LF cells were incubated in 80 µl of DMEM adding 20 μl of 5 mg/ml 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) solution (Beyotime, Nanjing, China) for 4 h. After the media removal, formazan crystals were dissolved in 100 μl DMSO/well, and the absorbance of the solution was measured at 490 nm. For colony-forming unit detection, the cells were pre-treated with Crystal Violet Staining Solution (Beyotime, Nanjing, China) for 10 min at 37˚C. For EdU and BrDu detection, the cells were pre-treated with EdU and BrDu working solution (Beyotime, Nanjing, China) for 2h at 37˚C. Finally, they were observed under the microscope (OLYMPUS, Nanjing, China).