2.9 | Mechanical stretching stimulation of LF
fibroblasts
LF fibroblasts were seeded in a special chamber (Beyotime, Beijing,
China) at 1×106 cells per chamber. The chamber had a
stretching apparatus, which cloud apply a cyclic stretch (5% and 10%
elongation) for cells. In this study, 10% elongation stretching was
applied for 6, 12 and 24 h (15 cycles/min, 37°C, 5% CO2). After
stretching stimulation, total RNA and protein were isolated to measure
expression of WISP-1.