2.9 | Mechanical stretching stimulation of LF fibroblasts
LF fibroblasts were seeded in a special chamber (Beyotime, Beijing, China) at 1×106 cells per chamber. The chamber had a stretching apparatus, which cloud apply a cyclic stretch (5% and 10% elongation) for cells. In this study, 10% elongation stretching was applied for 6, 12 and 24 h (15 cycles/min, 37°C, 5% CO2). After stretching stimulation, total RNA and protein were isolated to measure expression of WISP-1.