2.10 | RNA-seq analysis
Total RNA was isolated from LF fibroblasts collected from LDH and LSCS patients using RNeasy Plus Micro-Kit (Takara, Dalian, China) following the manufacturer’s instructions. Then, total RNA was provided to Nanjing medical university for preparing RNA-seq libraries and sequencing. The sequencing libraries were generated using the TruSeq RNA Sample Prep Kit (Illumina) as described by the manufacturer’s recommendations. The libraries were sequenced on two lanes of an Illumina HiSeq X-Ten for pair-end. EdgeR was used to distinguish the differentially expressing genes by pairwise comparisons.