Biosafety evaluation of CSL-gel
10 crucian carp with a length of 3-4 cm were placed into 1 L of pure water (pH=5.5) with different amounts of CSL-gel (the number of CSL-gel pieces per 1 L of water was 50, 100, 200, and 400). Pure tap water was used as a blank control, and each group has three repeats. The life activity and death of crucian carp were observed at 24 h intervals. Each treatment group has four replicates.