Construction of CML30 silencing vector
CML30 cDNA was amplified from leaf cDNA using primersCML -TRV-F and CML -TRV-R (Table 1 ). The fragment
was then inserted into VIGS vector Pash18 at the BamH I and Xhol I site.
Finally, the recombinant plasmids were introduced intoAgrobacterium tumefaciens EHA105. The empty vector, TRV-GFP was
used for control treatment.