Synthesis of CSL-gel
The SA, CaCl2, lentinan (LNT) and chitosan were used to
synthesis the CSL-ge following the method as described previously[25-26] : 1 g SA and 1 g LNT were dissolved in 125
mL of distilled water and stirred until completely dissolved.
Subsequently, the SA-LNT mixed solution was slowly dropped into 1000 mL
of aqueous CaCl2 (30 g/L) solution (stirred at 400
revolutions/min for 30 min) to form a hydrogel precursor (SL-ge)
immediately (loading efficiency[27] of 15.2% and
encapsulation percentage[27] of 89.4%). Next, 10
g/L aqueous chitosan solution was added to the surface of the SL-gel and
stirred for 2 h (400 revolutions/min) to form the CSL-gel. The CSL-gel
was washed three times with deionized water.