Overexpression of CML19 enhances the antiviral function
of Nicotiana benthamiana
To further confirm the antiviral function of CML19 in N.
benthamiana , we generated the CML19 overexpression construct
tagged with GFP and transiently overexpressed it in N.
benthamiana using agroinfiltration. Western blot detection showed that
the accumulation of GFP-CML19 protein was observed in the overexpression
leaves at two days after infiltration (Figure 5a ). Two days
after infiltration, all infiltrated leaves were inoculated with TMV-GFP.Figure 5c showed that at 2 dpi, strong GFP green fluorescent
signals were observed on the young leaves of the control plant compared
to that GFP:CML19- overexpressed plant. Limited GFP signals were
observed on the young leaves of the overexpression plant, while marked
signals were visualized on the young leaves of the control plant
(Figure 5c ). In addition, qPCR analysis showed that the
expression of TMV-CP in the GFP:00 plant was significantly higher
than that in the overexpression plant at 2 and 4 dpi (Figure 5
b ). These results indicated that overexpression of CML19inhibits TMV infection in N. benthamiana .