Overexpression of CML19 enhances the antiviral function of Nicotiana benthamiana
To further confirm the antiviral function of CML19 in N. benthamiana , we generated the CML19 overexpression construct tagged with GFP and transiently overexpressed it in N. benthamiana using agroinfiltration. Western blot detection showed that the accumulation of GFP-CML19 protein was observed in the overexpression leaves at two days after infiltration (Figure 5a ). Two days after infiltration, all infiltrated leaves were inoculated with TMV-GFP.Figure 5c showed that at 2 dpi, strong GFP green fluorescent signals were observed on the young leaves of the control plant compared to that GFP:CML19- overexpressed plant. Limited GFP signals were observed on the young leaves of the overexpression plant, while marked signals were visualized on the young leaves of the control plant (Figure 5c ). In addition, qPCR analysis showed that the expression of TMV-CP in the GFP:00 plant was significantly higher than that in the overexpression plant at 2 and 4 dpi (Figure 5 b ). These results indicated that overexpression of CML19inhibits TMV infection in N. benthamiana .