Construction of CML19 silencing vector
CML19 cDNA was amplified from leaf cDNA using primersCML -TRV-F and CML -TRV-R (Table S1 ). The fragment
was then inserted into VIGS vector Pash18 at the EcoR I and Xba Ⅰ site.
Finally, the recombinant plasmids were introduced intoAgrobacterium tumefaciens GV3101. The empty vector, TRV-GFP, was
used for the control treatment.