Synthesis of CSL-gel
The SA, CaCl2, lentinan (LNT) and chitosan were used to
synthesis the CSL-ge following the method as described previously (Xiang
et al., 2019, Gao C, 2009) : 1 g SA and 1 g LNT were dissolved in 125 mL
of distilled water and stirred until completely dissolved. Subsequently,
the SA-LNT mixed solution was slowly dropped into 1000 mL of aqueous
CaCl2 (30 g/L) solution (stirred at 400 revolutions/min
for 30 min) to form a hydrogel precursor (SL-ge) immediately (loading
efficiency (Lin Y H, 2008) of 15.2% and encapsulation percentage (Lin Y
H, 2008) of 89.4%). Next, 10 g/L aqueous chitosan solution was added to
the surface of the SL-gel and stirred for 2 h (400 revolutions/min) to
form the CSL-gel. The CSL-gel was washed three times with deionized
water.