The anti-TMV activity of CSL-gel mainly depends on the chitosan
shell and calcium ion
CSL-gel promotes the expression of CML19 and the silencing ofCML19 enhances TMV infection. To further determine thatCML19 induced by calcium ion released from the CSL-gel
contributes to the resistance against TMV infection, we generated the
CS-gel and S-gel without LNT to eliminate the role of LNT on the
anti-TMV activity. As shown in Figure 6a , at 2 dpi, the number
of green fluorescent spots in the inoculated leaves of N.
benthamiana treated with CS-gel was lower than that of the S-gel and
water-treated plants. qPCR analysis showed that the expression levels of
TMV-CP were significantly lower in the inoculated leaves ofN. benthamiana treated with CS-gel than that in the water and
S-gel-treated plants. At 7 dpi, the green fluorescence signals and the
number of TMV-CP transcripts of the water control group and S-gel
treatment group were significantly higher than that of CS-gel control.
However, there was no significant difference between the water-treated
and the S-gel-treated plans on TMV infection at 2 and 7 dpi. These
findings indicated that CML19 induced by CSL-gel also plays an
important role in anti-TMV.