Protein in silico methods
Protein information for RB protein (P06400, RB_HUMAN) and for hepatocyte growth factor receptor MET (P08581, MET_HUMAN) were obtained from UniProt database and from RCSB Protein Data Bank. GOR method version IV was used for secondary structure prediction [8]. Disorder prediction was performed by IUPred2A web server [9]. Eukaryotic linear motifs (ELM) were identified in the proteins by using ELM database [10]. Stability changes upon point mutations were predicted by I-Mutant 2.0 web server based on protein sequence, using default parameters [11]. Protein-protein interaction data were obtained from BioGRID [12] and STRING databases [13].