Figure legend
Figure 1. Conventional histological (H&E) characteristics of
patient tumor samples. A : Rb of the right eye bulb, B :
chondroblastic osteosarcoma of the left orbital bone, C :
osteoblastic osteosarcoma from the left tibia, D : osteoblastic
osteosarcoma, pulmonary metastasis. Arrows indicate retinoblasts, as
well as chondroblastic and osteoblastic propagation (magnification:
x20).
Figure 2. Immunohistochemistry of RB protein. A :
retinoblastoma (S1), B : left orbital bone (S3), C :
colorectal adenocarcinoma control. Arrows indicate the IHC-negative cell
nuclei of retinoblastic/ chondroblastic tumors and the positive nuclear
staining in adenocarcinoma cells (magnification: x40).
Figure 3. Structure and interactions of RB1. Schematic
represenation of full-length RB1 protein is shown. The structures of
N-terminal and central regions are represented, and the protein-protein
interactions of C-terminal region are also shown based on structural
data. Sequence of the C-terminal of region of RB1 is shown by black
color. Blue arrows show position of p.Gln850Ter mutation. The sequences
that are involved in protein-protein interactions are shown for each
coordinate file, these regions are highlighted by red in the complex
structures, as well.