Figure 9. srt1-4 hete affects the accumulation of transcripts regulated in the day
Reduced expression of SRT1 in a srt1-4 heterozygous mutant has a profound effect on the abundance of transcripts in the day. (a) Venn diagram showing the number of differentially expressed genes (FDR < 0.05) in srt1-4 hete , srt2-1 andsrt1-4hete x 2-1 mutants compared to Col-0 background in the day and the night as measured by RNAseq. Lists of differently expressed genes are given in Supplemental Table 5. (b) Venn diagrams showing the overlap between lists of up and down regulated genes in thesrt1-4 hete mutant at ZT6 and in the “white light” transcriptome from the EBI Gene Expression Atlas (Supplemental Table 3). There is a high degree of correspondence between transcripts up regulated in srt1-4 hete in the day and transcripts that are down regulated in a white light treatment
. Similarly, transcripts that are up regulated by whte lighIt are often in lower abundance in the mutant. (c)
The number of differentially expressed genes in common between thesrt1- 4 heterozygous mutants and plants treated with a particular treatments of white, red light and UV-A light, 1% sucrose or combined heat and salt stress, expressed as a percentage of the total number of differentially expressed genes in srt1-4 heterozygous mutants (see methods and Supplemental Table 3 for the data sets used). The differentially expressed genes in the srt1-4 heterozygous mutants are listed in Supplemental Table 5.