Figure 6. Nicotinamide increases the period of circadian rhythms
of leaf movement in parp, srt and parg mutants.
Circadian period of leaf movement in constant light in Col-0 and mutant
lines with and without dosing with 50 mM nicotinamide (nam) one and two
days before the start of imaging. Col-0 background minus (white) or plus
nicotinamide (grey). PARP mutants are blue, sirtuin mutants are
red and PARG mutants are green. Within each plot the darker colour shade
represents the mutant line treated with nicotinamide. All data are shown
with the mean indicated by a line. (a) Col-0 nrhy = 25,parp1-2 nrhy = 22, Col-0 + nic
nrhy = 12, parp1-2 + nic nrhy =
27 (b) Col-0 nrhy = 22, parp2-1nrhy = 11, Col-0 + nic nrhy = 25,parp1-2 + nic nrhy = 20 (c) Col-0 (as for (a))parp3-1 nrhy = 27, parp3-1 + nic
nrhy = 14 (d) Col-0 nrhy = 16,parp1-2x2-1x3-1 nrhy = 9, Col-0 + nic
nrhy = 23, parp1-2x2-1x3-1 + nic
nrhy = 22 (e) Col-0 nrhy = 12,srt1-4 hete nrhy = 33, Col-0 + nic
nrhy = 10, srt1-4 hete + nic nrhy= 21 (f) Col-0 nrhy = 15, srt2-1nrhy = 14, Col-0 + nic nrhy = 17,srt2-1 + nic nrhy = 18 (g) Col-0 (as for (e)),srt1-4 hete nrhy = 19, srt1-4 hete + nic
nrhy = 29 (h) parg2-1 nrhy = 31,parg2-1 + nic nrhy = 41 (i) Col-0 (as for (h)),
Col-0 nrhy = 48, parg1-1 nrhy =
22, Col-0 + nic nrhy = 36, parg1-1 + nic
nrhy = 21. Nrhy = the number of rhythmic
plants as estimated by FFT NLS.