Figure 4. srt1-4 insertion line has an embryo lethal phenotype.
(a) Gene structures of SRT1and SRT2. Confirmed insertion sites are indicated. Positions of relevant primers pairs are indicated. Gene F/R (black arrows) = primers positioned to flank insertion site, qPCR (green arrows) = primers positioned downstream of insertion site. Orange arrows indicate qPCR primers used by Liu et al., (2017) and red arrows indicate qPCR primers used in the study of Zhang et al., (2018) (b) Relative gene expression of SRT1 in srt1-1 , srt1-2 ,srt1-3 , srt1-4 hete and srt2-1 measured by qRT-PCR. N = 3 (c) Seeds of Arabidopsis SRT1 mutant alleles and Col- 0 background. srt1-4 heterozygous mutant has aborted seeds (white arrows) that are not observed in Col-0 background or srt1-2 ,srt2-1 , srt1-2x2-1 and srt1-3x2-1 . White bar indicates 1 mm. (d) Percentage seed set in srt1 mutants and threesrt1-4 complementation lines (COM). (Col-0 n = 516, srt1-1n = 542, srt1-2 n = 509, srt1-3 n = 501, srt1-4 hete n = 322, srt1-4 Complementation line #1 n = 602,srt1-4 Complementation line #2 n = 609 and srt1-4Complementation line #3 n = 586).