Figure 7. Circadian rhythms of circadian oscillator gene expression in parp1-2x2-1x3-1 mutants are delayed in the presence of nicotinamide.
Plants were entrained for nine days in 12L/12D before transfer into constant white light. On day seven, plants were transferred onto treatment media (10 mM nicotinamide or untreated control). Tissue samples (three biological replicates) were harvested every four hours in the third cycle of constant light. Abundance measured using qRT-PCR was normalised to UBQ10 . CCA1, TOC1 and PRR7peak abundance is delayed by nicotinamide treatment in both genotypes. Error bars represent standard deviation.