Delayed chlorophyll fluorescence
Seeds were sown in clusters of approximately 20 seeds, one cluster per well in a 10 cm square 25 well plate. The dividing walls of the plate were covered in black electrical tape, to reduce cross contamination of signal between wells. After 10 - 12 days of entrainment, plates were transferred into a NightSHADE LB 985 photon counting camera (Berthold Technologies), controlled by IndiGOTM software. Plants were grown in the camera box for one light/dark cycle before switching to constant light conditions for imaging. Illumination in the camera was maintained by light emitting diodes (LEDs) set to 24 % of maximum emission for red (660 nm) and blue (470 nm) wavelengths to provide light intensity 50 - 70 µmol m-2 s-2 at plant height. Delayed fluorescence (DF) was measured at a wavelength of 560 nm for 60 seconds post illumination every hour for five days. Images produced were analysed by IndiGOTM software, background corrected and detrended. Background corrected data was analysed using BRASS.