Figure 4. srt1-4 insertion line has an embryo lethal
phenotype.
(a) Gene structures of SRT1and SRT2. Confirmed insertion sites are indicated. Positions of
relevant primers pairs are indicated. Gene F/R (black arrows) = primers
positioned to flank insertion site, qPCR (green arrows) = primers
positioned downstream of insertion site. Orange arrows indicate qPCR
primers used by Liu et al., (2017) and red arrows indicate qPCR
primers used in the study of Zhang et al., (2018) (b) Relative
gene expression of SRT1 in srt1-1 , srt1-2 ,srt1-3 , srt1-4 hete and srt2-1 measured by qRT-PCR.
N = 3 (c) Seeds of Arabidopsis SRT1 mutant alleles and Col- 0
background. srt1-4 heterozygous mutant has aborted seeds (white
arrows) that are not observed in Col-0 background or srt1-2 ,srt2-1 , srt1-2x2-1 and srt1-3x2-1 . White bar
indicates 1 mm. (d) Percentage seed set in srt1 mutants and threesrt1-4 complementation lines (COM). (Col-0 n = 516, srt1-1n = 542, srt1-2 n = 509, srt1-3 n = 501, srt1-4
hete n = 322, srt1-4 Complementation line #1 n = 602,srt1-4 Complementation line #2 n = 609 and srt1-4Complementation line #3 n = 586).