Supplemental figure 8 srt1-4 hete and SRT1artificial micro RNA lines have similar effects on transcript
abundance.
(a) SRT1 abundance was measured by qRT-PCR in Col-0,srt1-1 , srt1-2 , srt1-3, srt2-1 T-DNA homozygous
mutants and in srt1-4 heterozygous T-DNA insertion lines, andSRT1 artificial micro RNA line #5 and #10. (b) Having confirmed
knockdown of SRT1 in srt1-4 heterozygous T-DNA insertion
lines and SRT1 artificial micro RNA line #5 and #10, transcript
abundance of the products of four genes was measured by qRT-PCR. n = 3
(b). These four genes were identified as being mis-regulated insrt1-4 heterozygous lines (AT2G37678, AT5G02200 andAT2G42870 ) and both srt1-4 heterozygous lines andsrt2-1 (AT1G70290 ) when analysed by RNA-seq (Supplemental
Table 5). Material for the qRT-PCR and RNAseq was grown and harvested
separately and the experiments were independent. All plants were
harvested during the day.