Figure 6. Nicotinamide increases the period of circadian rhythms of leaf movement in parp, srt and parg mutants.
Circadian period of leaf movement in constant light in Col-0 and mutant lines with and without dosing with 50 mM nicotinamide (nam) one and two days before the start of imaging. Col-0 background minus (white) or plus nicotinamide (grey). PARP mutants are blue, sirtuin mutants are red and PARG mutants are green. Within each plot the darker colour shade represents the mutant line treated with nicotinamide. All data are shown with the mean indicated by a line. (a) Col-0 nrhy = 25,parp1-2 nrhy = 22, Col-0 + nic nrhy = 12, parp1-2 + nic nrhy = 27 (b) Col-0 nrhy = 22, parp2-1nrhy = 11, Col-0 + nic nrhy = 25,parp1-2 + nic nrhy = 20 (c) Col-0 (as for (a))parp3-1 nrhy = 27, parp3-1 + nic nrhy = 14 (d) Col-0 nrhy = 16,parp1-2x2-1x3-1 nrhy = 9, Col-0 + nic nrhy = 23, parp1-2x2-1x3-1 + nic nrhy = 22 (e) Col-0 nrhy = 12,srt1-4 hete nrhy = 33, Col-0 + nic nrhy = 10, srt1-4 hete + nic nrhy= 21 (f) Col-0 nrhy = 15, srt2-1nrhy = 14, Col-0 + nic nrhy = 17,srt2-1 + nic nrhy = 18 (g) Col-0 (as for (e)),srt1-4 hete nrhy = 19, srt1-4 hete + nic nrhy = 29 (h) parg2-1 nrhy = 31,parg2-1 + nic nrhy = 41 (i) Col-0 (as for (h)), Col-0 nrhy = 48, parg1-1 nrhy = 22, Col-0 + nic nrhy = 36, parg1-1 + nic nrhy = 21. Nrhy = the number of rhythmic plants as estimated by FFT NLS.