Figure 5. parg 1-1 lengthens circadian period.
(a) Gene structures and confirmed insertion sites of PARG1 , withparg1 -1 (SALK_147805) allele, PARG2 and parg2 -1
(GK072_B04). Positions of relevant primers pairs are indicated. Gene
F/R (black arrows) = primers positioned to flank insertion site, qPCR
(green arrows) = primers positioned downstream of insertion site. (b-e)
Circadian rhythms in constant light of leaf movement and delayed
chlorophyll fluorescence in parg1-1 (b, c), parg2-1 (d, e)
and Col-0 background. (b, d) Mean Y pixel positions of leaf movement
from a single representative experiment and individual FFT-NLLS period
estimates and R.A.E values. (c, e) Delayed chlorophyll fluorescence.
Data are combined from separate experiments, (a) Col-0
nrhy = 158, parg1 -1 nrhy = 160
(b) Col-0 nrhy = 10, parg1- 1 nrhy= 9. (c) Col-0 nrhy = 83, parg2 -1
nrhy = 84 (d) Col-0 nrhy = 12,parg1- 1 nrhy = 11. Error bars represent standard
deviation. Nrhy = the number of rhythmic plants as
estimated by FFT NLS.