Supplemental figure 7 Abundance of selected transcripts measured by qRT-PCR in sirtuin mutants compared to RNAseq data.
Genes encoding transcripts that were mis-regulated in srt1-4 heteRNA-seq were selected for further analysis by independent qRT-PCR in an attempt to confirm the major findings. At2G36970 was selected because it has a large decrease in abundance in RNAseq in the mutant.AT5G55580 and AT5G55540 were selected to represent transcripts that had smaller changes in abundance. AT5G62720, AT4G11830 and AT2G15880 were selected to represent large increases in abundance changes in srt1-4 hete the RNAseq data. (a, c) qRT-PCR of Col-0, srt1-4 hete, srt2-1 and microRNA lines SRT1amiR#5 and SRT1amiR#10 (b,d) RNA-sequencing data from Col-0, srt1-4 hete and , srt2-1 as number of reads. Samples were obtained in the day (a,b, ZT6) and night (c,d) ZT18. The qRT-PCR and RNAseq studies were performed independently on material grown and harvested separately.