Figure 9. srt1-4 hete affects the accumulation of
transcripts regulated in the day
Reduced expression of SRT1 in a srt1-4 heterozygous mutant
has a profound effect on the abundance of transcripts in the day. (a)
Venn diagram showing the number of differentially expressed genes (FDR
< 0.05) in srt1-4 hete , srt2-1 andsrt1-4hete x 2-1 mutants compared to Col-0 background in the day
and the night as measured by RNAseq. Lists of differently expressed
genes are given in Supplemental Table 5. (b) Venn diagrams showing the
overlap between lists of up and down regulated genes in thesrt1-4 hete mutant at ZT6 and in the “white light”
transcriptome from the EBI Gene Expression Atlas (Supplemental Table 3).
There is a high degree of correspondence between transcripts up
regulated in srt1-4 hete in the day and transcripts that are down
regulated in a white light treatment
. Similarly, transcripts that are up regulated by whte lighIt are often
in lower abundance in the mutant. (c)
The number of differentially expressed genes in common between thesrt1- 4 heterozygous mutants and plants treated with a particular
treatments of white, red light and UV-A light, 1% sucrose or combined
heat and salt stress, expressed as a percentage of the total number of
differentially expressed genes in srt1-4 heterozygous mutants
(see methods and Supplemental Table 3 for the data sets used). The
differentially expressed genes in the srt1-4 heterozygous mutants
are listed in Supplemental Table 5.