Sirtuin mutants do not have altered circadian rhythms
SRT1 and 2 transcript abundance was weakly rhythmic in 24 hours of constant light (Supplemental Figure 1) but we found no evidence that SIRTUINS participate in the regulation of circadian period in Arabidopsis. Neither the srt2-1 knock out, the srt1-4 SRT1heterozygous knock down or double mutants of those had any significant effect on the circadian period of leaf movement rhythms (Figure 3e,f,g, Col-0 24.1 ± 2.2 h, srt1-4 SRT1 24.7 ± 2.4 h, srt2-1 24.0 ± 1.6 h; srt1-4 SRT1 x 2-1 24.2 ± 3.4 h P > 0.3). Similarly, knock down of SRT1 and knockout of SRT2 was without effect on circadian rhythms of delayed chlorophyll fluorescence (Supplemental Figure 3e,f, Col-0 24.2 ± 3.1 h, srt1-4 hete 24.4 ± 3.3 h, srt2-1 24. ± 0.6 h P > 0.4).