Photoperiodic flowering time screening
Sixteen plants per line were sown into soil trays with 24 cells with the
positions being allocated randomly. The seeds were stratified for three
days at 4 °C before transfer to 20 °C and
100 µmol m-2 s-1 in either long day
(LD) conditions (16 h L:8 h D) or short day (SD) conditions (8 h L: 16 h
D). Seed trays were moved regularly to prevent any positional effects on
plant growth. The time to bolt and the number of rosette leaves at the
time of bolting were recorded. Bolting time was measured as the number
of days from sowing when the inflorescence stem was approximately 5 mm
tall.