Figure 8. FLC is misexpressed in parp mutants, which have delayed flowering in short day photoperiods
(a, b) Venn diagrams of the number of transcripts differentially expressed (FDR < 0.05) as measured by RNAseq inparp1-2 , parp2-1 , parp3-1 andparp1-2x2-1x3-1 mutants compared to Col-0 background in (a) day at ZT6 and (b) night at ZT18. (c ,d) Transcript abundance of FLCmeasured by qRT-PCR in Col-0, parp1-2 , parp2-1 ,parp3-1 and parp1-2x2-1x3-1 in the (c) day and (d) night. qRT-PCR normalised to UBQ10 . Seedlings were grown under 12 h L/ 12 h D in white light on 1/2 MS agar plates for two weeks and sampled during the day (ZT6) and night (ZT18). Growth, harvesting, RNA extraction and qRT-PCR analysis for FLC was performed independently of the RNAseq experiments. Error bars represent the standard deviation. n = 3. (e, f) Flowering time as measured by the emergence of bolt and numbers of rosette leaves at bolting of Col-0, andparp1-2, parp2-1, parp3-1, parp1-2x2-1x3-1 under (e) long (16 h L:8 h D) or (f) short day (8 h L:16 h D). The number of rosette leaves and bolting time were recorded when the emerging bolt was 5 mm high. Dots represent the individual plants and the black horizontal bars the mean. In long photoperiods n  = 12 for each genotype and in short photoperiods n  = 15 for each genotype. The Kruskal-Wallis-one-way analysis of variance was used to determine if there was any overall difference among the 5 genotypes. Dunn’s method was then used to test the significance of pairwise comparisons between Col-0 and each mutant. Single asterisks indicate P ≤ 0.05. Col-0 (white) parp1-2(yellow), parp2-1 (red), parp3-1 (green) andparp1-2x2-1x3-1 (blue)