Supplemental figure 2 Characterisation of PARP T-DNA mutant alleles.
(a) Gene structures and confirmed insertion sites of PARP1 , withparp1 -2 (SAIL_514F10) allele, PARP2 and parp2 -1 (GK380_E06) allele and PARP3 with the parp3 -1 (SALK108_092) allele. See text for precise positions. Positions of relevant primers pairs are indicated. Gene F/R (black arrows) = primers positioned to flank insertion site, qPCR (green arrows) = primers positioned downstream of insertion site. (b) PCR products from gDNA of triple mutant and Col-0 background plants confirm a homozygous tripleparp1-1x2-1x3-1 mutant. Gene-specific forward and reverse primers flanking the predicted insertion site amplify products from Col-0 gDNA but not gDNA from homozygous T-DNA insertion mutant gDNA. T-DNA insertion mutants amplify a product using LB primer and a gene specific primer. F = Forward primer, R= reverse primer, LB = T-DNA left border primer. (c) Relative abundance of PARP1 , PARP2 andPARP3 transcripts in Col-0 and parp1-2x2-1x3-1 measured by qRT-PCR. N = 3.