Figure 1 . FT-IR spectra of P. Nagi kernel oil in the
frequency range 4000-500cm-1
3.4 In vitro biological evaluation
3.4.1 Antioxidant effect ofP. nagi kernel oil
The radical scavenging ability of P. nagi kernel oil was
evaluated in the conventional system of DPPH⸱. Firstly, we added 40μLP. nagi kernel oil to
different concentrations of DPPH respectively and studied the scavenging
ability to optimize the best experimental conditions. The results
(Figure 2 ) showed that the optimized DPPH concentration is
0.036mg/mL and the scavenging ability is 86.56%; Then, we also added
different amounts of P. nagi kernel oil to 5mL DPPH solution to
optimize the optimal concentration of P. nagi kernel oil with the
highest scavenging ability. The results (Figure 3 ) showed that
the P. nagi kernel oil exhibited the scavenging ability in a
concentration-dependent manner from 5μL to 40μL and exhibited the
highest scavenging ability at 40μL with 85.56%. Then, we tested the
scavenging ability of P. nagi kernel oil at the optimized
conditions: 5 mL of 0.036mg/mL DPPH solution, P. nagi kernel oil
40μL, the three times of scavenging abilities are: 85.07%, 85.67% and
84.80%; the average scavenging ability is 85.18%.