2.1 ∣ Samples
Samples of WWS were maintained at the Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming, China (N25˚3’47”, E102˚45’18”, H1,950m). For de novo sequencing and analysis, we collected ~1,500 individuals of female larvae in first instar from one colony into three micro-tubes and sink in liquid nitrogen for DNA isolation and whole genome sequencing. For DNA methylation and analysis, we collected 500 female and male individuals, respectively, to make sure that the samples are sufficient for total DNA isolation and the following genetic analyses. The samples for detecting hormone and RNA extraction were collected every 3 days from first instar larvae to aging adults and stored in liquid nitrogen until used.