2.1 ∣ Samples
Samples of WWS were maintained at the Research Institute of Resource
Insects, Chinese Academy of Forestry, Kunming, China (N25˚3’47”,
E102˚45’18”, H1,950m). For de novo sequencing and analysis, we
collected ~1,500 individuals of female larvae in first
instar from one colony into three micro-tubes and sink in liquid
nitrogen for DNA isolation and whole genome sequencing. For DNA
methylation and analysis, we collected 500 female and male individuals,
respectively, to make sure that the samples are sufficient for total DNA
isolation and the following genetic analyses. The samples for detecting
hormone and RNA extraction were collected every 3 days from first instar
larvae to aging adults and stored in liquid nitrogen until used.