Lipase activity assay
Lipase activity assay buffer was prepared with sodium phosphate, sodium chloride, 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate hydrate (CHAPS) and 4-methylumbelliferyl decanoate (MUD4) at pH 7.4. After the mixing, the plate was analyzed in the fluorescence detector, Tecan Spark. The maximum excitation/emission wavelengths were determined to be 340 nm and 450 nm. The kinetic measurement was reported over 1.5 hours with 45 seconds interval. The slope of fluorescence over time is directly proportional to the hydrolytic activity of the sample used within the linear measuring range. The hydrolytic activity allows determination of lipase content in the sample.