Lipase activity assay
Lipase activity assay buffer was prepared with sodium phosphate, sodium
chloride, 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate
hydrate (CHAPS) and 4-methylumbelliferyl decanoate (MUD4) at pH 7.4.
After the mixing, the plate was analyzed in the fluorescence detector,
Tecan Spark. The maximum excitation/emission wavelengths were determined
to be 340 nm and 450 nm. The kinetic measurement was reported over 1.5
hours with 45 seconds interval. The slope of fluorescence over time is
directly proportional to the hydrolytic activity of the sample used
within the linear measuring range. The hydrolytic activity allows
determination of lipase content in the sample.