STRING network for significant proteins in all treatments
A protein network map was created visually representing the response ofO. mossambicus gill proteins to hypersaline conditions regardless of time of exposure or position relative to the critical threshold using all 161 proteins significantly regulated in all treatments. The majority (122) of accession numbers matched to STRING IDs, and 86 were connected to at least one other protein. The network map of all connected proteins contains many proteins involved in the electron transport chain (ETC), with 36 of the 86 total proteins being a subunit of one of the five ETC complexes (Figure 4). Core members of the glycolysis and tricarboxcylic acid (TCA) cycle pathways accounted for another 17 nodes of this network map. Only three of the proteins in the network map were downregulated, Na+/K+-ATPase α-1 isoform X4, Alkylglycerone phosphate synthase, and NH4+ transporter Rh-B. One of the clusters which is highly connected included two isoforms of acetyl-CoA acyltransferase, two isoforms of aldehyde dehydrogenase 6-A1, two trifunctional proteins, enoyl-CoA δ isomerase 1, and electron transfer flavoprotein β. Another important set of nodes on this map are the two isoforms of IMPase, one of which was the most highly upregulated protein in many treatments.