STRING networks for pejus and pessimus range physiological
states
All treatments 85g/kg and greater represent pessimum range salinities
given the ultimate MP endpoint at this salinity. Network analysis was
run for the 78 proteins shared by 14-day acclimations and extended
salinity treatments at 85g/kg and 105g/kg and compared with the networks
for the 87 proteins unique to the extended 75g/kg treatment. Networks
were identified with greater than three nodes and classified using
ontological term enrichment. Pejus range regulation consisted of a
network of mostly upregulated proteins with enrichment in amino acid
metabolism and oxidoreductase terms including lactate dehydrogenase
(LDH) and glycogen synthase, a network of mixed up and down-regulation
of structural proteins, especially myosin subunits, and downregulation
of proteasome proteins (Figure 5A). Pessimum range networks contained
specific upregulated mitochondrial proteins, with subunits in the
cytochrome b-c1 complex as central members, and downregulation of
extracellular matrix proteins (ECM) with several collagen subunits
(Figure 5B).