Feeding
In the 14-day acclimation, fish at 21g/kg appeared to increase feeding compared to the control, although this trend was not significant by the conclusion of the experiment (Figure 1E). The 55g/kg treatment feeding behavior was very similar to the control. Both the 85g/kg and 105g/kg treatments decreased towards complete cessation of feeding as the target salinity was reached. For extended constant salinity at 85g/kg, feeding dropped to near zero over the 14-day acclimation period before rising slightly and finally falling back towards zero as fish reached MP. At 105g/kg constant salinity feeding dropped to near zero during acclimation and remained near zero until MP. At 75g/kg, feeding dropped to near zero during the acclimation period but recovered to near the feeding rate of the handling control, although at 12-weeks feeding was still significantly lower (p = 0.0093) than the control (Figure 2C).