Correlation analysis
The ratio of blood osmolality, body condition, time at high salinity (>65g/kg) and salinity at time of sample collection versus the control value for each treatment used in the proteomic analysis was compared against the FC for this treatment was used to compare treatments based on linear correlation coefficient (r2). To account for differences between the short-term and long-term acclimation controls due to the high feed rate and age difference between these two, the controls were compared using the same procedure as each treatment to control with MSstats 3.1 and any proteins which were significantly different between controls were not considered. Following this filtering step, proteins with r2 greater than 0.9 were identified and further refined to only those proteins which were significantly regulated in at least one treatment. To determine functional groups which were most correlated with a given parameter, the list of accession numbers of proteins which met the above conditions were searched on the STRING database and networks identified.