Fitness of the HI lineage on fresh and pre-infected cucumber
In the first-generation life table, net reproductive rate
(R 0) of the HI lineage was only 8.92 on fresh
cucumber versus 20.44 on pre-infected cucumber (p =0.003); the
intrinsic rate (r ) was 0.15 on fresh cucumber versus 0.23 on
pre-infected cucumber (p <0.001); the finite rate of
increase (λ ) and the generation time (T ) were 1.16 and
14.20 on fresh cucumber, compared to 1.25 and 13.07 on pre-infected
cucumber, respectively (p <0.001 and p =0.002,
respectively) (Figure 1A). In life table of the third generation (F3),R 0, r , λ and T were 2.11,
0.09, 1.10 and 11.48 on fresh cucumber respectively, compared to 57.6,
0.42, 1.52 and 10.07 on pre-infected cucumber, and the differences
between fresh and pre-infected cucumber were all significant
(p <0.001) (Figure 1A). We transferred the nymphs
produced by the HI lineage who was cultured on pre-infected cucumber for
two generations to fresh cucumber, and we surprisingly found that they
performed as well as the CU lineage did on fresh cucumber in the four
parameters: R 0 (57.06 vs 65.38, p =0.342),r (0.38 vs 0.37, p =0.897) and λ (1.46 vs 1.45,p =0.879) (Figure 1A). The survival and fecundity curves clearly
showed that the HI lineage performed much better on pre-infected
cucumber than on fresh cucumber (Figure 1B), and the difference between
pre-infected and fresh cucumber became more obvious in the third
generation (Figure 1B). The survival and fecundity curves also showed
that the HI lineage with feeding experience on pre-infected cucumber (HI
adapted) performed as well as the CU lineage on fresh cucumber (Figure
1C).