Figure 4 . Effects of phenanthridine derivatives on
hydroquinone-treated C57BL/6 mice. As described in the Methods, the
C57BL/6 mice were coated with 5% hydroquinone for 50 consecutive days
to establish a mouse model of vitiligo. Subsequently, the animals were
acclimatized for 1 week and randomly divided into eight groups,
including one negative control group, one 8-MOP group, three
HCJA121-treated groups, and three HCJA404-treated groups, each group has
12 mice (n=12). The 8-MOP group was administered 8-MOP (4.25 mg/kg), and
the drug-treated groups were administered HCJA121 or HCJA404 at one of
three doses (L: 0.0425 mg/kg, M: 0.425 mg/kg and H: 4.25 mg/kg). In
addition, 12 normal mice were established as the blank control. At the
end of the 30-day treatment, the blood was collected retro-orbitally
from the mice, and dorsal skin samples were isolated from the animals
and stained with HE. (a). Recovery effects of phenanthridine derivatives
on skin lesions in hydroquinone-treated C57BL/6 mice; (b). Compared to
mice from the NC group, the tyrosinase in the blood of the drug-treated
mice recovered remarkably, and the MDA content decreased significantly.
(c) Skin histomorphology changes in the treated mice are presented.
Photomicrography indicated that HCJA121 and HCJA404 are excellent for
promoting the repair of hydroquinone-damaged melanin in epidermal basal
layer cells. The asterisk indicates the thickened stratum corneum, the
black arrow indicates a layer of darker-purple granules, the yellow
arrow indicates the increased spinal cell layer in the epidermal layer,
and the red arrows indicate the hair follicle sebaceous glands;
magnification, 200×. Data shown are means ± S.E.M; n = 12 mice per
group. Significantly different as indicated (*P < 0.05,
**P < 0.01, ***P < 0.001, and
****P < 0.0001).