3.2.1. Marker efficiency analysis
A total of 104 bands and 99 polymorphic bands were amplified from 24 SSR
primers, and the percentage of polymorphic loci was 95.19%. Ninety-six
pairs of alleles were amplified, the largest N as(eight) were amplified using primers YM06 and YM08, with an average of
four alleles per primer. The effective N a ranged
from 1 to 6.429, with an average of 2.488. The mean I , observed
heterozygosity (H o), and expected heterozygosity
(H e) were 0.864, 0.422, and 0.441, respectively.
The H ranged from 0 to 0.845, with an average of 0.439 (Table 5).
From the 49 SRAP primers, 29 were
selected to produce distinct and stable bands, and 215 bands were
amplified, in which 212 were polymorphic, accounting for 98.60% of the
total bands. The number of bands amplified by each primer ranged from
two to 11, with an average of 7.4. The polymorphic bands amplified by
primers F1R2, F3R7, and F7R4 were up to 11. The number of available
alleles ranged from 1.072 to 1.519, with an average of 1.342, and the
mean H was 0.216. I varied from 0.145 to 0.472, with an
average of 0.344 (Table 6).