3.2.1. Marker efficiency analysis
A total of 104 bands and 99 polymorphic bands were amplified from 24 SSR primers, and the percentage of polymorphic loci was 95.19%. Ninety-six pairs of alleles were amplified, the largest N as(eight) were amplified using primers YM06 and YM08, with an average of four alleles per primer. The effective N a ranged from 1 to 6.429, with an average of 2.488. The mean I , observed heterozygosity (H o), and expected heterozygosity (H e) were 0.864, 0.422, and 0.441, respectively. The H ranged from 0 to 0.845, with an average of 0.439 (Table 5).
From the 49 SRAP primers, 29 were selected to produce distinct and stable bands, and 215 bands were amplified, in which 212 were polymorphic, accounting for 98.60% of the total bands. The number of bands amplified by each primer ranged from two to 11, with an average of 7.4. The polymorphic bands amplified by primers F1R2, F3R7, and F7R4 were up to 11. The number of available alleles ranged from 1.072 to 1.519, with an average of 1.342, and the mean H was 0.216. I varied from 0.145 to 0.472, with an average of 0.344 (Table 6).