Figures Legends
Figure 1 : Monosaccharide profiles of Panicum maximum in different climate conditions: Control (C), Elevated Temperature (eT), Elevated CO2 (eC) and combined treatments (eT+eC). Mean values ± SD (n=3) marked with different letters above bars indicate statistical differences between treatments (p≤0.05, Tukey’s test).
Figure 2 : Principal Component Analysis of Panicum maximum cell wall composition in different climate conditions: Control (C), Elevated Temperature (eT), Elevated CO2 (eC) and combined treatments (eT+eC).
Figure 3 : Lignin monomers of Panicum maximum in different climate conditions: Control (C), Elevated Temperature (eT), Elevated CO2 (eC) and combined treatments (eT+eC). CW: Cell Wall. Mean values ± SD (n=3) marked with different letters above bars indicate statistical differences between treatments (p≤0.05, Tukey’s test).
Figure 4 : Carbohydrates content in leaves of Panicum maximum . Reducing sugars (A) and starch (B). Treatments: Control (C), Elevated Temperature (eT), Elevated CO2 (eC) and combined treatments (eT+eC). Mean values ± SD (n=3) marked with different letters above bars indicate statistical differences between treatments (p≤0.05, Tukey’s test).
Figure 5 : Glucose (A) and Xylan (B) conversion of untreatedPanicum maximum after enzymatic saccharification at 72 h and 2% solid content. Treatments: Control (C), Elevated Temperature (eT), Elevated CO2 (eC) and combined treatments (eT+eC). Mean values ± SD (n=3) marked with different letters above bars indicate statistical differences between treatments (p≤0.05, Tukey’s test).
Figure 6 : Binding isotherm of FTCM-depletion assay probes: GC3a (A), CC17 (B), OC15 (C) and CC27 (D) to C, eT, eC and eT+eC substrates at 25°C in a 20 mM Tris–HCl (pH 7.5) buffer containing 20 mM NaCl, 5 mM CaCl2. Treatments: Elevated Temperature (eT), Elevated CO2 (eC) and combined treatments (eT+eC).
Figure 7: Correlation between accessibility (total cellulose accessibility (A) as detected by FTCM probes (GC3a + CC17) and total hemicelluloses accessibility (B) as detected by FTCM probes (OC15 + CC27)) and percent glucan/xylan hydrolysis.