Comparative proteomic analysis and plant salinity response of two quinoa
genotypes
Abstract
The aim of this study was to investigate the effect of NaCl salinity (0,
100 and 300 mM) on the individual response of the quinoa varieties
Kcoito (Altiplano Ecotype) and UDEC-5 (Sea-level Ecotype) with
physiological and proteomic approaches. UDEC-5 showed an enhanced
capacity to withstand salinity stress compared to Kcoito. In response to
salinity, we detected overall the following differences between both
genotypes: Toxicity symptoms, plant growth performance, photosynthesis
performance and intensity of ROS-defense. We found a mirroring of these
differences in the proteome of each genotype. Among the 700 protein
spots reproducibly detected, 24 exhibited significant abundance
variations between samples. These 24 proteins were involved in energy
and carbon metabolism, photosynthesis, ROS scavenging and
detoxification, stress defense and chaperone functions, enzyme
activation and ATPases. A specific set of proteins predominantly
involved in photosynthesis and ROS scavenging showed significantly
higher abundance under high salinity (300 mM NaCl). The adjustment was
accompanied by a stimulation of various metabolic pathways to balance
the supplementary demand for energy or intermediates. However, the more
salt-resistant genotype UDEC-5 presented a beneficial and significantly
higher expression of nearly all stress-related altered enzymes than
Kcoito. Salinity, halophyte, quinoa, proteomic, photosynthesis,
antioxidant, salt resistance, oxidative stress.