2.5 Histological analysis of liver tissue samples
Formalin‐fixed, paraffin‐embedded liver tissue sections were stained
with haematoxylin, eosin and sirius red. Liver necrosis, bile duct
proliferation and hepatic inflammation were quantified in a blinded
manner on a 1‐ to 5‐point scale using a Leica DM1000 microscope. Sirius
red‐ stained sections from each animal were observed at low
magnification and analysed using ImageJ software to calculate the
percentage of hepatic fibrous area.