Healthy Subjects Active SLE PR-SLE p
N = 29 N = 15 N = 15
CD4+
PD-1+ % 31.40 (24.80 – 40.60) 43.40 (32.10 – 52.30) 42.90 (31.80 – 63.60) < 0.01*, †
Tim-3+ % 17.90 (11.60-32.75) 11.90 (7.99-53.40) 7.85 (3.18-32.30) 0.13
CD38+HLA-DR+ (Activated) % 4.01 (2.56 – 5.72) 6.86 (5.09 – 12.1) 8.18 (4.66 – 10.5) < 0.01***, †††
POP 4 2B4+ % 2.79 (0.85 – 8.69) 5.27 (2.47 – 11.10) 5.05 (3.02 – 7.97) 0.10
POP 5 CD38+PD-1+ % 9.09 (5.63 – 13.25) 15.20 (6.78 – 24.60) 14.30 (8.57 – 24.10) 0.01†
POP 6 CD38+HLA-DR+PD-1+ % 1.02 (0.62 – 1.92) 3.48 (1.59 – 4.20) 2.28 (1.47 – 3.96) < 0.001***, †††
CD57+ % 3.13 (1.79 – 6.25) 4.19 (3.66 – 8.57) 5.22 (2.12 – 10.4) 0.24†
EOMES+ % 1.59 (0.87 – 2.49) 1.39 (0.48 – 2.87) 5.24 (3.38 – 14.70) < 0.001‡‡, †††
T-bet+ % 4.93 (2.28 – 8.32) 1.69 (1.30 – 4.02) 5.05 (3.23 – 9.16) 0.01‡
POP 0 CM CD57+ % 3.90 (1.42 – 6.58) 4.90 (1.45 – 39.60) 12.00 (5.41 – 35.60) 0.01†
POP 1 CD45RO+T-bet+ % 4.08 (1.64 – 5.48) 1.68 (0.42 – 2.83) 10.20 (1.74 – 30.50) < 0.01†, ‡‡
POP 4 EM CD27-CD45RO+ % 2.36 (1.32 – 4.38) 2.15 (0.45 – 12.50) 3.16 (1.29 – 7.42) 0.57
CD8+
PD-1+ % 27.9 (23.9 – 35.4) 27.4 (13.6 – 42.9) 38.6 (30.3 – 49.1) 0.02†
Tim-3+ % 3.16 (1.77-7.34) 2.58 (2.23-3.95) 3.09 (0.64-6.22) 0.84
CD38+HLA-DR+ (Activated) % 6.28 (2.72 – 8.07) 9.38 (6.30 – 17.3) 12.5 (8.47 – 18.7) < 0.01 ††
CD38+HLA-DR+PD-1+ (Exhausted) % 1.24 (0.88 – 1.94) 4.69 (1.70 – 10.2) 4.38 (2.09 – 9.19) < 0.001***, †††
POP 0 2B4+ % 58.9 (49.3 – 68.3) 46.7 (27.1 – 60.8) 68.4 (49.7 – 79.0) 0.02‡
POP 2 CD38+PD-1+ % 6.24 (4.38 – 9.23) 12.0 (8.26 – 26.9) 13.6 (10.7 – 27.7) < 0.0001**, †††
POP 7 CD38+HLA-DR+2B4+ % 2.23 (1.17 – 3.06) 7.11 (3.66 – 11.80) 8.97 (4.00 – 14.80) < 0.0001**, †††
CD57+ % 28.0 (21.5 – 36.6) 38.0 (31.4 – 45.4) 37.6 (26.2 – 52.4) 0.02††
EOMES+ % 12.9 (6.78 – 24.8) 8.13 (2.33 – 20.5) 37.6 (24.9 – 53.2) < 0.001††, ‡‡‡
T-bet+ % 32.8 (18.9 – 44.2) 26.4 (10.7 – 44.2) 47.8 (23.0 – 70.1) 0.09‡
POP 3 CD27+CD45RO+T-bet+ % 2.06 (1.00 – 6.02) 2.70 (0.76 – 5.91) 5.75 (3.51 – 10.10) 0.02†
POP 5 DP CD4+CD8+CD27+CD45RO+ % 0.15 (0.10 – 0.30) 0.20 (0.13 – 0.36) 0.28 (0.23 – 0.47) 0.15
POP 6 CD27-CD45RO-CD57+T-bet+EOMES+ % 0.81 (0.37 – 3.06) 1.42 (0.33 – 6.27) 4.54 (0.88 – 9.78) 0.02†
POP 7 DN CD4-CD8-CD57+T-bet+ % 0.23 (0.11 – 0.49) 0.32 (0.07 – 0.53) 0.37 (0.21 – 0.68) 0.35
Bold type represents populations identified by traditional manual gating. Pop populations that were inferred from FlowSOM Heat Map are in cursive. Results are shown in Median with Interquartile Range, and Kruskal Wallis test was used to perform comparisons between groups. Mann Whitney U test was performed to compare between two groups as a post Hoc test and are represented by the following symbols. * Healthy Subjects vs Act SLE. * p < 0.05. ** p < 0.01. *** p < 0.001. † Healthy Subjects vs PR-SLE. † p < 0.05. †† p < 0.01. ††† p < 0.001. ‡ Act-SLE vs PR-SLE. ‡ p < 0.05. ‡‡ p < 0.01. ‡‡‡ p < 0.001. Bold type represents populations identified by traditional manual gating. Pop populations that were inferred from FlowSOM Heat Map are in cursive. Results are shown in Median with Interquartile Range, and Kruskal Wallis test was used to perform comparisons between groups. Mann Whitney U test was performed to compare between two groups as a post Hoc test and are represented by the following symbols. * Healthy Subjects vs Act SLE. * p < 0.05. ** p < 0.01. *** p < 0.001. † Healthy Subjects vs PR-SLE. † p < 0.05. †† p < 0.01. ††† p < 0.001. ‡ Act-SLE vs PR-SLE. ‡ p < 0.05. ‡‡ p < 0.01. ‡‡‡ p < 0.001. Bold type represents populations identified by traditional manual gating. Pop populations that were inferred from FlowSOM Heat Map are in cursive. Results are shown in Median with Interquartile Range, and Kruskal Wallis test was used to perform comparisons between groups. Mann Whitney U test was performed to compare between two groups as a post Hoc test and are represented by the following symbols. * Healthy Subjects vs Act SLE. * p < 0.05. ** p < 0.01. *** p < 0.001. † Healthy Subjects vs PR-SLE. † p < 0.05. †† p < 0.01. ††† p < 0.001. ‡ Act-SLE vs PR-SLE. ‡ p < 0.05. ‡‡ p < 0.01. ‡‡‡ p < 0.001. Bold type represents populations identified by traditional manual gating. Pop populations that were inferred from FlowSOM Heat Map are in cursive. Results are shown in Median with Interquartile Range, and Kruskal Wallis test was used to perform comparisons between groups. Mann Whitney U test was performed to compare between two groups as a post Hoc test and are represented by the following symbols. * Healthy Subjects vs Act SLE. * p < 0.05. ** p < 0.01. *** p < 0.001. † Healthy Subjects vs PR-SLE. † p < 0.05. †† p < 0.01. ††† p < 0.001. ‡ Act-SLE vs PR-SLE. ‡ p < 0.05. ‡‡ p < 0.01. ‡‡‡ p < 0.001. Bold type represents populations identified by traditional manual gating. Pop populations that were inferred from FlowSOM Heat Map are in cursive. Results are shown in Median with Interquartile Range, and Kruskal Wallis test was used to perform comparisons between groups. Mann Whitney U test was performed to compare between two groups as a post Hoc test and are represented by the following symbols. * Healthy Subjects vs Act SLE. * p < 0.05. ** p < 0.01. *** p < 0.001. † Healthy Subjects vs PR-SLE. † p < 0.05. †† p < 0.01. ††† p < 0.001. ‡ Act-SLE vs PR-SLE. ‡ p < 0.05. ‡‡ p < 0.01. ‡‡‡ p < 0.001. Bold type represents populations identified by traditional manual gating. Pop populations that were inferred from FlowSOM Heat Map are in cursive. Results are shown in Median with Interquartile Range, and Kruskal Wallis test was used to perform comparisons between groups. Mann Whitney U test was performed to compare between two groups as a post Hoc test and are represented by the following symbols. * Healthy Subjects vs Act SLE. * p < 0.05. ** p < 0.01. *** p < 0.001. † Healthy Subjects vs PR-SLE. † p < 0.05. †† p < 0.01. ††† p < 0.001. ‡ Act-SLE vs PR-SLE. ‡ p < 0.05. ‡‡ p < 0.01. ‡‡‡ p < 0.001.