R. pedestris.
Figure 2. Identification of chromosomes in Riptortus
pedestris. (A) DNA interaction heatmap generated in Hi-C interaction
analysis. The shading gradient represents the chromosome interactions.
(B) Chromosomic staining of female and male salivary gland cells inR. pedestris . The blue signal indicates the condensed
chromosomes.
Figure 3. Landscape of the assembled Riptortus pedestrisgenome. From the outside in, the first circle shows the 6 chromosomes.
The second circle showed the GC contents. The third and fourth circle
showed the female and male MRPM value, respectively. The fifth circle
showed the ratio of MRPM between female and male. The sixth circle
showed the transcriptional reads from 37 different development stages.
All data was calculated on a 1-Mb scale.
Figure 4. Analysis of Serratia marcescens Rip1. (A)
Landscape of the assembled Serratia marcescens Rip1 genome. From
the outside in, the first circle showed the nucleotide sequence
positions (in Mb). The second circle showed the virulence factors and
antibiotic-resistance genes in the genome. The third and fourth circle
showed the predicted genes that transcribed in clockwise and
anticlockwise, respectively. The
fifth circle showed the GC contents on a 10-Kb scale. rfaF,
ADP-heptose-LPS heptosyltransferase II; gpt, glycerol-3-phosphate
transporter; PmrB, two-component system sensor histidine kinase BasS;
alaS, aminocoumarin resistant gene; kdpE, two-component system response
regulator KdpE ; fbpC, Iron (III) ABC transporter; galE, UDP-glucose
4-epimerase; msbA, Lipid transporter ATP-binding/permease; mfd,
transcription-repair coupling factor; shuU, ABC transport system; SRT-2,
class C beta-lactamase; cysB, aminocoumarin resistant transcriptional
regulator CysB; flg, flagella synthesis genes; katG ,
catalase/peroxidase HPI ; omp36 , porin OmpC; fepG, Iron-enterobactin
ABC transporter permease; lpxD, UDP-3-O-(3-hydroxymyristoyl) glucosamine
N-acyltransferase; AAC(6’)-Ic, aminoglycoside N-acetyltransferase
AAC(6’)-Ic. (B) Phylogenetic relationship between Serratiaspecies.
Figure 5.Phylogenetic analysis ofRiptortus pedestris with other insect species. The phylogenetic
position of R. pedestris was determined based on 963 single copy
genes with 356,827 reliable sites. D. melanogaster was used to
root the tree. 1:1:1 indicates single copy genes, and N:N:N indicates
multicopy genes across 14 insect species. The Sternorrhyncha,
Auchenorrhyncha, and Heteroptera indicate suborder‐specific genes,
respectively. The estimated species divergence time is illustrated at
the bottom of the phylogenetic tree. Gene family expansion (red) and
contraction (green) are illustrated at the branches and nodes of the
tree.
Figure 6. Genome synteny between Triatoma rubrofasciataand Riptortus pedestris. Synteny analysis demonstrated fusion or
fission events between the two bug species.