Lp(a)-Overexpressing Mouse experiments
Lp(a) transgenic mice were produced as previously described2. Briefly, human apo(a) Yeast Artificial Chromosomes
(YAC) transgenic mice were created by insertion of human apo(a) YAC,
including the 110 kb apo(a) gene, 70 kb apo(a)-like gene, and the 270 kb
genomic DNA (YAC DNA) containing 5′-prime of plasminogen gene. Lp(a)
transgenic mice were created by mating human apo(a) transgenic and human
apoB transgenic Friend Virus B (FVB) mice and selection of double
homo-transgenic mice. Four-week-old Lp(a) transgenic (N=10) and FVB
non-transgenic control (N=10) mice were fed a high cholesterol diet for
6 weeks then sacrificed. The experiments were approved by the Ethical
Committee for Animal Experiments of the Tokushima University Graduate
School of Medicine.