CCHF diagnosis
Two blood samples of at least 2 mL each were collected from every
patient with suspected CCHF upon hospital admission. After 30 minutes of
coagulation, the samples were centrifuged at 2000 rpm for 5 min and the
serum was transferred to separate tubes. One serum sample from each
patient was transported under appropriate conditions to the Erzurum
Regional Public Health Laboratory, our regional reference center, for
serologic and virologic tests. CCHF was diagnosed based on specific
anti-IgM antibody detected by immunofluorescence assay (IFA) and/or
polymerase chain reaction (PCR) positivity.