2.6.1 Outcrossing rates from progeny arrays
Outcrossing rates were estimated from nine heterostylous families with a
mean number of 10 seedlings per family from D07 (dimorphic heterostylous
population) and seven homostylous families with 7.86 ± 1.06 (mean ± SE)
seedlings per family from M01 (fully homostylous population). Both
maternal and seedling plants were genotyped with the same
microsatellites as in section 2.4. Multi-locus
(tm ) and single-locus (ts )
outcrossing rates and their associated standard errors were estimated
with MLTR v.3.4 (Ritland,
2002),
using 10000 bootstrap resamplings of maternal families. We used a
one-sided Wilcoxon signed-rank test to asses whether homostylous
individuals have a lower outcrossing rate than distylous individuals.
Moreover, the germination rate of heterostylous and homostylous families
was used as a fitness proxy to estimate the strength of inbreeding
depression (δ) as 1 – (WHO /WHE ) (Goodwillie, Kalisz, & Eckert,
2005)
where WHO and WHE are the
mean germination rates of homostyles and heterostyles, respectively.