Figure 3.-  Variation of CYPᵀ detected in 44 individuals of Primula vulgaris (17 short-styled and 27 homostylous individuals) from ten natural populations sampled for this study, plus two CYPᵀ alleles (indicated with *) from two homostylous individuals previously reported as CYPᵀ SLH1 and SLH2 by Li et al. (2016); CYPᵀ is a 1587 bp long hemizygous gene that comprises five exons (base-pair lengths of each exon are indicated in parentheses). A) Graphical representation of the nine CYPᵀ alleles with different types of mutations in CYPᵀ2–9: vertical lines, white triangles, and black triangles represent point mutations, deletions, and insertions, respectively, with positions of each mutation reported above each exon; one synonymous, and five nonsynonymous mutations, two deletions, and one insertion were found. B) Haplotype network of the nine CYPᵀ alleles: each circle (i.e., node in the network) represents a different CYPᵀ allele (CYPᵀ1–9, listed next to each circle), with circle size proportional to the number of homostylous (grey) and short-styled (black) individuals carrying that allele (reported inside the circle); nodes are connected by solid lines representing the lowest number of mutational steps between alleles, in this case always a single mutational step indicated by a single dash across the line, except for CYPᵀ-1 and CYPᵀ-5 connected by two mutational steps; grey dashed lines represent alternative connections among haplotypes with two mutational steps between alleles, indicated by a double dash across the line.