2.6.1 Outcrossing rates from progeny arrays
Outcrossing rates were estimated from nine heterostylous families with a mean number of 10 seedlings per family from D07 (dimorphic heterostylous population) and seven homostylous families with 7.86 ± 1.06 (mean ± SE) seedlings per family from M01 (fully homostylous population). Both maternal and seedling plants were genotyped with the same microsatellites as in section 2.4. Multi-locus (tm ) and single-locus (ts ) outcrossing rates and their associated standard errors were estimated with MLTR v.3.4 (Ritland, 2002)⁠⁠, using 10000 bootstrap resamplings of maternal families. We used a one-sided Wilcoxon signed-rank test to asses whether homostylous individuals have a lower outcrossing rate than distylous individuals. Moreover, the germination rate of heterostylous and homostylous families was used as a fitness proxy to estimate the strength of inbreeding depression (δ) as 1 – (WHO /WHE ) (Goodwillie, Kalisz, & Eckert, 2005)⁠⁠ where WHO and WHE are the mean germination rates of homostyles and heterostyles, respectively.