Figure Legends
Figure 1. Cell growth of
suspension adapted Vero cells in IHM03 (A) and MDXK (B) medium in a 1 L
bioreactor in batch mode. The concentration of the main metabolic
substrates (glucose, L-glutamine) and by-products (lactate, ammonia) are
given in mM.
Figure
2. rVSV-ZEBOV infection of suspension adapted Vero cells at different
MOIs ranging from 0.001 to 0.1 in 125 mL shake flask in IHM03 medium at
a cell density of 1 × 106 cells/mL. Infectious viral
titers expressed as TCID50/mL are plotted against the
time post-infection. Bars represent the mean of the three independent
samples ± standard deviation.
Figure
3. rVSV-ZEBOV infection of suspension adapted Vero cells at different
cell densities ranging from 1 × 106 to
4 × 106 cells/mL in 125 mL shake flask in IHM03
medium. Infectious viral titers expressed as TCID50/mL are plotted
against the time post-infection. Bars represent the mean of the three
independent samples ± standard deviation.
Figure
4. rVSV-ZEBOV (A), rVSV-HIV (B) and
rVSVInd-msp -SF-Gtc (C)
infections of suspension adapted Vero cells cultivated in either IHM03
or MDXK medium at an MOI of 0.01, in 125 mL shake flask at a cell
density of 1 × 106 cells/mL. Infectious viral titers
expressed as TCID50/mL are plotted against the time
post-infection. Bars represent the mean of the three independent samples
± standard deviation.
Figure 5. Comparison of
the production of rVSV-ZEBOV in suspension adapted Vero cells cultivated
in either IHM03 or MDXK medium in a 1 L bioreactor in batch mode.
Infectious viral titers expressed as TCID50/mL and the
number of viral genomes in VG/mL are plotted against the time
post-infection.
Figure
6. Comparison of the production of rVSV-HIV in suspension adapted Vero
cells cultivated in either IHM03 or MDXK medium in a 1 L bioreactor in
batch mode. Infectious viral titers expressed as
TCID50/mL and the number of viral genomes in VG/mL are
plotted against the time post-infection.
Figure 7. Comparison of
the production of
rVSVInd-msp -SF-Gtc in
suspension adapted Vero cells cultivated in either IHM03 or MDXK medium
in a 1 L bioreactor in batch mode. Infectious viral titers expressed as
TCID50/mL and the number of viral genomes in VG/mL are
plotted against the time post-infection.