Species delineation
To estimate the number of species present in the dataset, we used a
molecular species delimitation method, the Poisson tree process (PTP)
(Zhang, Kapli, Pavlidis, & Stamatakis, 2013), conducted on the bPTP
webserver (http://species.h-its.org/ptp). We applied the same
methodology as in Morek & Michalczyk (2020), constructing the tree
based on concatenated COI+ITS-2 sequences in MrBayes. The results were
then compared with morphological data and genetic distances, to verify
the putative species identified by bPTP. In parallel to genetic
delineation, we attempted to identify species using the latest
diagnostic key to the genus (Morek, Gąsiorek, Stec, Blagden, &
Michalczyk, 2016). Species that could not be identified are designated
as “sp. nov.” followed by population code (international country code
and sample number). If there were two species from the same population,
an additional number/letter was added to discriminate between them. In
order to aid navigation through phylogeny, all species identified and
delineated in the present study are given running numbers, from #1 to
#64.