Small interfering RNA (siRNA) transfection and RNA sequencing
Small interfering RNA (siRNA) for GRP78 and negative control was sourced
from Ruibo (Guangzhou, China). siRNA targeting GRP78 or negative control
siRNA was transfected into RA FLSs using transfection reagent (polyplus)
according to the manufacturer’s instructions. For RNA sequencing, TNF-α
or IL-1β activated RA FLSs were treated with AZM for 24 hours. Then,
total RNA was extracted by TRIzol method to construct a cDNA library for
RNA transcriptome sequencing by LC-BIO Technologies CO., LTD (Hangzhou,
China).