Authorship contribution
Eric HJALMARSSON and Marianne PETRO have made substantial contributions in acquisition and data analysis. Eric HJALMARSSON have been involved in drafting the manuscript. All authors have been involved in conception, design, data interpretation and manuscript revising. All authors have read and approved the final manuscript.
Figure 1, Notch1-4 on T-cells in nasal mucosa and blood .Fraction of CD4 T-cells expressing Notch in A: peripheral blood and B: nasal mucosa. C: Flow cytometry gating strategy for detection of Notch expression. Patients with allergic rhinitis (AR), (n=9), healthy controls (HC), (n=11). Horizontal lines represent the mean value and SEM. p<0.01= **.
Figure 2, Expression of Notch ligands on epithelial cells and neutrophils A: Fraction of epithelial cells expressing Notch ligands. Median fluorescence intensity of B: JAG-1 and C: DLL-1. D: Fraction of neutrophils in nasal mucosa expressing Notch ligands. Median fluorescence intensity of E: JAG-1 and F: DLL-1. G: Fraction of neutrophils in blood expressing Notch ligands. H: Median fluorescence intensity of I: JAG-1 and J: DLL-1. Patients with allergic rhinitis (AR), (n=9), healthy controls (HC), (n=11). Horizontal lines represent the mean value and SEM. p<0.05= *, p<0.01= **.
1. Coquet JM, Rausch L, Borst J. The importance of co-stimulation in the orchestration of T helper cell differentiation. Immunol Cell Biol. 2015;93(9):780-788.
2. Shi L, Ma Y, Zheng C, Zhang Q. The effect of blocking Notch signaling by gamma-secretase inhibitor on allergic rhinitis. Int J Pediatr Otorhinolaryngol. 2017;98:32-38.
3. Tindemans I, Lukkes M, de Bruijn MJW, et al. Notch signaling in T cells is essential for allergic airway inflammation, but expression of the Notch ligands Jagged 1 and Jagged 2 on dendritic cells is dispensable. J Allergy Clin Immunol. 2017;140(4):1079-1089.
4. Xia M, Harb H, Saffari A, Sioutas C, Chatila TA. A Jagged 1-Notch 4 molecular switch mediates airway inflammation induced by ultrafine particles. J Allergy Clin Immunol. 2018;142(4):1243-1256 e1217.
5. Tindemans I, Peeters MJW, Hendriks RW. Notch Signaling in T Helper Cell Subsets: Instructor or Unbiased Amplifier? Front Immunol.2017;8:419.
6. Polak D, Hafner C, Briza P, et al. A novel role for neutrophils in IgE-mediated allergy: Evidence for antigen presentation in late-phase reactions. J Allergy Clin Immunol. 2019;143(3):1143-1152 e1144.