Authorship contribution
Eric HJALMARSSON and Marianne PETRO have made substantial contributions
in acquisition and data analysis. Eric HJALMARSSON have been involved in
drafting the manuscript. All authors have been involved in conception,
design, data interpretation and manuscript revising. All authors have
read and approved the final manuscript.
Figure 1, Notch1-4 on T-cells in nasal mucosa and blood .Fraction of CD4 T-cells expressing Notch in A: peripheral blood and B:
nasal mucosa. C: Flow cytometry gating strategy for detection of Notch
expression. Patients with allergic rhinitis (AR), (n=9), healthy
controls (HC), (n=11). Horizontal lines represent the mean value and
SEM. p<0.01= **.
Figure 2, Expression of
Notch ligands on epithelial cells and neutrophils A: Fraction of
epithelial cells expressing Notch ligands. Median fluorescence intensity
of B: JAG-1 and C: DLL-1. D: Fraction of neutrophils in nasal mucosa
expressing Notch ligands. Median fluorescence intensity of E: JAG-1 and
F: DLL-1. G: Fraction of neutrophils in blood expressing Notch ligands.
H: Median fluorescence intensity of I: JAG-1 and J: DLL-1. Patients with
allergic rhinitis (AR), (n=9), healthy controls (HC), (n=11). Horizontal
lines represent the mean value and SEM. p<0.05= *,
p<0.01= **.
1. Coquet JM, Rausch L, Borst J. The importance of co-stimulation in the
orchestration of T helper cell differentiation. Immunol Cell
Biol. 2015;93(9):780-788.
2. Shi L, Ma Y, Zheng C, Zhang Q. The effect of blocking Notch signaling
by gamma-secretase inhibitor on allergic rhinitis. Int J Pediatr
Otorhinolaryngol. 2017;98:32-38.
3. Tindemans I, Lukkes M, de Bruijn MJW, et al. Notch signaling in T
cells is essential for allergic airway inflammation, but expression of
the Notch ligands Jagged 1 and Jagged 2 on dendritic cells is
dispensable. J Allergy Clin Immunol. 2017;140(4):1079-1089.
4. Xia M, Harb H, Saffari A, Sioutas C, Chatila TA. A Jagged 1-Notch 4
molecular switch mediates airway inflammation induced by ultrafine
particles. J Allergy Clin Immunol. 2018;142(4):1243-1256 e1217.
5. Tindemans I, Peeters MJW, Hendriks RW. Notch Signaling in T Helper
Cell Subsets: Instructor or Unbiased Amplifier? Front Immunol.2017;8:419.
6. Polak D, Hafner C, Briza P, et al. A novel role for neutrophils in
IgE-mediated allergy: Evidence for antigen presentation in late-phase
reactions. J Allergy Clin Immunol. 2019;143(3):1143-1152 e1144.