Figure 1
Effect of media volume on tissue formation. (A) Biochemical accumulation of cartilaginous constituents (GAG, collagen and DNA). (B) Histological (sulphated proteoglycan stain: safranin-O ; collagen stain:sirius red ) and immunohistochemical (collagen I and collagen II) appearance of the developed tissue constructs. Data presented as mean ± SEM, normalized to 1 mL control (n=6/group);a significantly different (p<0.05); all scale bars: 150 µm.