Figure 2
Effect of media volume on metabolic pathway activity and stabilization
of HIF-1α. (A) Intracellular pyruvate (n=4/group), PHD2 activity
(n=3/group), and HIF-1α expression (n=3/group). Biochemical data
normalized to 1 mL. (B) Selected gene targets of HIF-1α (SOX9, PDK1,
GLUT1) (n=3/group). qPCR expression calculated using the
ΔΔCT method. Data presented as mean ± SEM;a significantly different from 1 mL
(p<0.05); b significantly different from 1
mL and 2 mL (p<0.05); * significantly
different from all other groups (p<0.05).