3.3.1 Dip Netting versus electrofishing sampling method
3’ Tag-Seq data identified only 3 of the 39,212 genes recovered in both
tested groups (<<1%) with different expression
across all tissues between dip netting and electrofishing sampling
(adjusted p- value < 0.05). When different tissues were
analyzed separately, we observed no difference in gene expression
between these two sampling methods for blood samples (27,118 genes),
less than 1% for gill samples (16 of 20,465 genes with adjustedp- value < 0.05), and ~1.7% (155 of
9,201 genes with adjusted p -value < 0.05) in muscle
samples. Whole mRNA-Seq (NEB) data (available only for blood samples)
confirmed the data obtained for 3’ Tag-Seq with no gene showing
different expression between the two sampling methods (out of 33,236
genes recovered) (Supporting Information Table S3).