3.3.1 Dip Netting versus electrofishing sampling method
3’ Tag-Seq data identified only 3 of the 39,212 genes recovered in both tested groups (<<1%) with different expression across all tissues between dip netting and electrofishing sampling (adjusted p- value < 0.05). When different tissues were analyzed separately, we observed no difference in gene expression between these two sampling methods for blood samples (27,118 genes), less than 1% for gill samples (16 of 20,465 genes with adjustedp- value < 0.05), and ~1.7% (155 of 9,201 genes with adjusted p -value < 0.05) in muscle samples. Whole mRNA-Seq (NEB) data (available only for blood samples) confirmed the data obtained for 3’ Tag-Seq with no gene showing different expression between the two sampling methods (out of 33,236 genes recovered) (Supporting Information Table S3).