Patients selection and data collection
Patients affected by NB treated from March 2005 to March 2019 at the
Bambino Gesù Children Hospital of Rome, Italy, were retrospectively
enrolled. The patient’s disease was staged according to age at the time
of diagnosis (< or ≥ 18 months), INSS [2] and the INRGSS
[3]. The amplification of MYCN gene was determined by fluorescent in
situ hybridization (FISH) [25]. Clinical and pathological data of
all cases were retrieved from the medical records available in the
institutional archives. The inclusion criteria were defined as follows:
(i) patients affected by NB; (ii) patients who underwent MYCN gene
determination; (iii) patients with at least 6 months of follow up (iv)
patients images had to be stored in Dicom format.