2.7 qRT-PCR
The expression profiles of all 16 DEMI among the multi-omics interaction
network and the selected 70 DEG representing biological processes
induced by hypoxia were further analyzed using qRT-PCR. qRT-PCR methods
were the same as description in our previous article
(Zhang et al. 2019). All primers
are shown in Table S1 . 2-△△CT method was used
to calculate the expression levels, and the data were subjected to
statistical significant analysis (P < 0.05) using SPSS
22.0 (Student’s t-test).