2.7 qRT-PCR
The expression profiles of all 16 DEMI among the multi-omics interaction network and the selected 70 DEG representing biological processes induced by hypoxia were further analyzed using qRT-PCR. qRT-PCR methods were the same as description in our previous article (Zhang et al. 2019). All primers are shown in Table S1 . 2-△△CT method was used to calculate the expression levels, and the data were subjected to statistical significant analysis (P < 0.05) using SPSS 22.0 (Student’s t-test).