2.5 Transcriptome, soluble sugar and phytohormones analyses
Anthers were divided into three stages based on the similarity of response to 4 d DS during phenotypic evaluation experiment-stage I, [PMC-MEI]: were anthers at pollen mother cell and younger (PMC) and MEI stages with length ≤ 2.8mm; stage 2, [TED-VUM]: were anthers at TED, EUM and VUM stages with lengths ≥ 4mm to ≤ 6.4mm; stage 3, [BIN-MP]: were anthers at transition to pollen mitosis 1 and early binucleate (BIN) to mature pollen (MP) stages with length >7mm. On 4 d of DS, samples from WW and DS plants were collected for each stage, immediately frozen in liquid nitrogen and stored at -75°C until used.