Figure 6. Comparison of sucrose and starch metabolism in
developing anthers exposed to drought stress. (A) Expression
analysis of genes involved in sucrose and starch metabolism, and sugar
transport based on RNA-Seq data. (B) Sucrose, glucose, and
fructose contents in anthers of DS plants. Anthers of equivalent
developmental stages were sampled from WW and DS plants 4 d after
drought stress. Values are mean ± SD (n = 3 replications,
24 plants each). *p <0.05; **p <0.01;
***p < 0.001 (t-test). (C) Starch accumulation
in tomato anthers determined by staining with Iodine Potassium Iodide
(IKI) solution. WW , anthers from well-watered plants;DS , anthers from drought-stressed plants; MEI , anthers
at meiotic stage; TED , anthers at tetrad stage; EUM ,
anthers at early uninucleate microspore stage; VUM , anthers at
vacuolated uninucleate microspore stage; BIN , anthers
binucleate stage; MP , anthers at mature pollen stage.