3.1. Performance of the quadruple zebrafish microfluidic device
Compared to our single-fish device reported previously[16], the design modifications to the quadrupole-fish device in this paper had an impact on the electric signal distribution and the fluid flow through the device, meriting characterization.
The indirect flow offered a convenient way of steering the fish from the main channel to fill the open TRs. An example of how each fish was trapped in place is seen in trap A in Fig. 2. The quadruple-fish device functionality and success rate were assessed based on the testing time per fish, loading and orientation efficiencies defined by Eq. (3), (4) and (5), respectively.
\(Testing\ time\ per\ fish=\frac{Loading\ time+60\ s\ recovery\ period+20\ s\ stimulation\ interval}{Number\ of\ fish\ oriented\ tail-first\ in\ the\ device}\ \)