3.1. Panx1a Function Impacts Molecular and Behavioural Changes Induced by 6-OHDA Treatment in 5dpf Zebrafish Larvae
The initial evidence for a potential involvement of Panx1a in zebrafish larvae electric-induced response was detected whenPanx1a-/- larvae responded with shorter RD and higher TBF compared to the panx1a+/+ group. The effect of 6-OHDA on locomotor activity was evaluated by examining the behavioral phenotypes of RD and TBF of larvae exposed to electrical stimulus (Fig. 2A and 2B, with all p-values presented in Table 1). Exposure to 6-OHDA for 72hrs caused a 68% and 50% decrease in the RD and TBF of panx1a+/+ control larvae. The results were consistent with previously published data, where 6-OHDA treatment was associated with mobility deficits[44], [45]. However, these changes in the locomotor activity were absent inpanx1a-/- larvae after 6-OHDA treatment, suggesting that 6-OHDA treatment does not induce locomotor deficits in 5dpf larvae in the absence of Panx1a functions. We concluded that Panx1 channels and 6-OHDA might function through similar signaling pathways, so that in the absence of Panx1 the 6-OHDA target is disrupted as well[6], [46].