3.1. Panx1a Function Impacts Molecular and Behavioural Changes
Induced by 6-OHDA Treatment in 5dpf Zebrafish Larvae
The initial evidence for a potential involvement of Panx1a in zebrafish
larvae electric-induced response was detected whenPanx1a-/- larvae responded with shorter RD and
higher TBF compared to the panx1a+/+ group. The
effect of 6-OHDA on locomotor activity was evaluated by examining the
behavioral phenotypes of RD and TBF of larvae exposed to electrical
stimulus (Fig. 2A and 2B, with all p-values presented in Table 1).
Exposure to 6-OHDA for 72hrs caused a 68% and 50% decrease in the RD
and TBF of panx1a+/+ control larvae. The
results were consistent with previously published data, where 6-OHDA
treatment was associated with mobility deficits[44], [45].
However, these changes in the locomotor activity were absent inpanx1a-/- larvae after 6-OHDA treatment,
suggesting that 6-OHDA treatment does not induce locomotor deficits in
5dpf larvae in the absence of Panx1a functions. We concluded that Panx1
channels and 6-OHDA might function through similar signaling pathways,
so that in the absence of Panx1 the 6-OHDA target is disrupted as
well[6], [46].