Fig. 3. Morphological and molecular characterization of transgenic switchgrass plants overexpressing bacterial NfsI. (A) Representative switchgrass plants from control (Ctrl) and transgenic (NfsI_OE-02 and -14) switchgrass plants are shown. (B) Quantitative real-time PCR analysis of NfsI transcript abundances in control and transgenic switchgrass plants. The control plants were generated with the pANIC6B empty vector. Stems at the R1 stage were collected. The expression level of switchgrass PvUbiq2 was used as the reference for normalization. Values are mean ± SE (n = 3).