2,4-DNT and DNTS uptake by control and transgenic switchgrass
plants
The control and transgenic switchgrass plants were propagated through
tiller-splitting. The uniform
switchgrass plants at E2 stage were selected and transplanted in 200 mL
1/2 MS liquid medium without sugar
and supplied with 20 mg·L-1 2,4-DNT and 1
g·L-1 DNTS, respectively. The switchgrass plants were
grown in a growth chamber at 25 °C with a 16-hour light/8-hour dark
photoperiod for 6 days, while 50 μL of liquid medium were sampled each
day. The contents of 2,4-DNT and DNTS were quantified by HPLC, as
previously described (Xu et al., 2019).