Fig. 6. Transcriptome analysis of transgenic switchgrass plants
responses to 2,4-DNT treatment. (A) Venn diagram and intersecting sets
analysis for upregulated and downregulated genes among the control and
transgenic switchgrass plants with and without 2,4-DNT treatment. CP-UT,
control plants without 2,4-DNT treatment; CP-T, control plants with 20
mg·L-1 2,4-DNT treatment; TP-UT, transgenic plants
(NfsI_OE-02 and -14) without 2,4-DNT treatment; TP-T, transgenic plants
(NfsI_OE-02 and -14) with 20 mg·L-1 2,4-DNT treatment
for 2 days. The horizontal bars represent different intersections of
four sets of samples (I-VIII). The vertical bars represent the gene
number of the intersection. (B) Gene ontology term enrichment of
differentially expressed genes (DEGs) of intersection IV and VI. (C)
KEGG pathway enrichment of DEGs in intersection VI. (D) KEGG pathway
enrichment of DEGs in intersection IV. The control and transgenic
(NfsI_OE-02 and -14) plantlets were exposed to 0 and 20
mg·L-1 2,4-DNT, respectively. After 2 days treatment,
total RNAs were extracted and subjected to transcriptome analysis
through RNA-sequencing. Each sample included three vegetatively
propagated copies. Values are mean ± SE (n = 3).