Statistical analysis
Switchgrass plants were propagated by transferring the same number of
tillers into each pot. Three copies of each line were grown in 1-gallon
pots. In this study, the control
switchgrass plants and two representative NfsI overexpressing
transgenic lines were selected for further investigation of root length,
ROS contents, 2,4-DNT and DNTS uptake, as well as transcriptome
profiling. Each sample included at least three vegetatively propagated
copies. Data from each treatment were subjected to an analysis of
variance (ANOVA). The significance of the treatments was tested at thep ≤0.05 level. Standard errors were provided in all tables and
figures as appropriate. All statistical analyses were performed using
the SPSS package (SPSS Inc., Chicago, IL, USA).