Fig. 6. Transcriptome analysis of transgenic switchgrass plants responses to 2,4-DNT treatment. (A) Venn diagram and intersecting sets analysis for upregulated and downregulated genes among the control and transgenic switchgrass plants with and without 2,4-DNT treatment. CP-UT, control plants without 2,4-DNT treatment; CP-T, control plants with 20 mg·L-1 2,4-DNT treatment; TP-UT, transgenic plants (NfsI_OE-02 and -14) without 2,4-DNT treatment; TP-T, transgenic plants (NfsI_OE-02 and -14) with 20 mg·L-1 2,4-DNT treatment for 2 days. The horizontal bars represent different intersections of four sets of samples (I-VIII). The vertical bars represent the gene number of the intersection. (B) Gene ontology term enrichment of differentially expressed genes (DEGs) of intersection IV and VI. (C) KEGG pathway enrichment of DEGs in intersection VI. (D) KEGG pathway enrichment of DEGs in intersection IV. The control and transgenic (NfsI_OE-02 and -14) plantlets were exposed to 0 and 20 mg·L-1 2,4-DNT, respectively. After 2 days treatment, total RNAs were extracted and subjected to transcriptome analysis through RNA-sequencing. Each sample included three vegetatively propagated copies. Values are mean ± SE (n = 3).