Fig. 4. Effect of 2,4-DNT on root length and ROS content of
control and transgenic switchgrass plants. (A) Root length of the
control and transgenic switchgrass plantlets were measured after 2,4-DNT
treatment. (B) In situ detection of ROS in roots of control and
transgenic switchgrass plantlets by DAB staining. (C) ROS content in
roots of the control and transgenic switchgrass plantlets were measured
after 2,4-DNT treatment. The control and transgenic (NfsI_OE-02 and
-14) switchgrass plantlets were exposed to 0, 2, and 20
mg·L-1 2,4-DNT. After 14 days of treatment, the
photographs of roots stained with NBT were taken. Root length as well as
ROS content were also measured. Each sample included six vegetatively
propagated copies. Values are mean ± SE (n = 3).