Fig. 1. Metabolites of 2,4-DNT and DNTS transformed by
NfsI in vitro. HPLC analysis of metabolites of 2,4-DNT (A) and
DNTS (B) transformed by the purified recombinant NfsI in vitro .
The proposed pathway for the reduction of 2,4-DNT (C) and DNTS (D) by
NfsI. Peak 1, 2/4-hydroxyl amino-4/2-mononitrotoluene (HAMNT); Peak 2,
2/4-amino-4/2-mononitrotoluene (AMNT); Peak 3, a novel compound derived
from HAMNT; Peak 4, 4-hydroxyl
amino-4/2-mononitrotoluene-3-SO3-(HAMNTS).