2,4-DNT and DNTS uptake by control and transgenic switchgrass plants
The control and transgenic switchgrass plants were propagated through tiller-splitting. The uniform switchgrass plants at E2 stage were selected and transplanted in 200 mL 1/2 MS liquid medium without sugar and supplied with 20 mg·L-1 2,4-DNT and 1 g·L-1 DNTS, respectively. The switchgrass plants were grown in a growth chamber at 25 °C with a 16-hour light/8-hour dark photoperiod for 6 days, while 50 μL of liquid medium were sampled each day. The contents of 2,4-DNT and DNTS were quantified by HPLC, as previously described (Xu et al., 2019).