Fig. 3. Morphological and molecular characterization of
transgenic switchgrass plants overexpressing bacterial NfsI. (A)
Representative switchgrass plants from control (Ctrl) and transgenic
(NfsI_OE-02 and -14) switchgrass plants are shown. (B) Quantitative
real-time PCR analysis of NfsI transcript abundances in control
and transgenic switchgrass plants. The control plants were generated
with the pANIC6B empty vector. Stems at the R1 stage were collected. The
expression level of switchgrass PvUbiq2 was used as the reference
for normalization. Values are mean ± SE (n = 3).