Fig. 1. Metabolites of 2,4-DNT and DNTS transformed by NfsI in vitro. HPLC analysis of metabolites of 2,4-DNT (A) and DNTS (B) transformed by the purified recombinant NfsI in vitro . The proposed pathway for the reduction of 2,4-DNT (C) and DNTS (D) by NfsI. Peak 1, 2/4-hydroxyl amino-4/2-mononitrotoluene (HAMNT); Peak 2, 2/4-amino-4/2-mononitrotoluene (AMNT); Peak 3, a novel compound derived from HAMNT; Peak 4, 4-hydroxyl amino-4/2-mononitrotoluene-3-SO3-(HAMNTS).